This article deals with the developments of optical microscopy towards nanoscopy. Basic concepts of the methods implemented to obtain spatial super-resolution are described, along with concepts related to the study of biological systems at the molecular level. Fluorescence as a mechanism of contrast and spatial resolution will be the starting point to developing a multi-messenger optical microscope tunable down to the nanoscale in living systems. Moreover, the integration of optical nanoscopy with scanning probe microscopy and the charming possibility of using artificial intelligence approaches will be shortly outlined.

中文翻译：

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光学纳米显微镜

本文讨论光学显微镜向纳米显微镜的发展。描述了获得空间超分辨率的方法的基本概念，以及与分子水平的生物系统研究相关的概念。荧光作为对比度和空间分辨率的机制将成为开发可在生命系统中调谐至纳米尺度的多信使光学显微镜的起点。此外，光学纳米显微镜与扫描探针显微镜的集成以及使用人工智能方法的迷人可能性将很快得到概述。