The effect of varying boron concentrations on in vivo pollen germination and tube penetration in four different apple cultivar crosses among ‘Golden Delicious,’ ‘Red Delicious,’ ‘Gala’ and ‘Fuji’ was evaluated. Boric acid (H₃BO₃) with two different concentrations: 1000 and 2000 mg L⁻¹, was sprayed on the foliage long before the onset of flowering. Pollen–pistil interaction vis-à-vis pollen germination and tube penetration was evaluated using fluorescence microscopy technique, 72 and 120 h after manual pollination. Among different crosses, ♀Golden delicious × Red delicious♂ treated at 1000 mg L⁻¹ concentration of boric acid showed maximum (27.39%) pollen germination and pollen tubes at different levels inside the style. In control without boron, only 6.49% germination was observed. Interaction of boron conc. × time depicted maximum pollen germination and tube number 120 hap. The present study demonstrates that foliar application of boron beyond 1000 mg L⁻¹ on different apple cultivars reduces germination of pollen and development of pollen tubes down the style.

评估了不同硼浓度对“金冠”，“红冠”，“嘎拉”和“富士”四个苹果品种杂交后体内花粉萌发和试管穿透的影响。在开花开始前很长时间，将两种浓度的硼酸（H ₃ BO ₃）：1000和2000 mg L ^-1喷在叶子上。在人工授粉后72和120 h，使用荧光显微镜技术评估了花粉与雌蕊对花粉萌发和花粉管穿透的相互作用。在不同的杂交中，“金黄色×红色美味”以1000 mg L ^-1处理 硼酸浓度最高，花粉发芽率最高（27.39％），花粉管在花粉中的不同含量水平。在不含硼的对照中，仅观察到6.49％的发芽。硼浓缩物的相互作用。×时间描绘了最大花粉萌发和120 hap的管数。本研究表明，在不同的苹果品种上叶面施用超过1000 mg L ^-1的硼会降低花粉的发芽和花粉管的发育。