The sterile insect technique (SIT), a benign pest control strategy, is currently undergoing a substantial renewal for controlling the Queensland fruit fly (Q-fly), a major horticultural pest in Australia. Production and delivery of high-quality flies is fundamental to effective SIT. While efforts are commonly made to improve production at a factory level (both quantity and quality), the methods and conditions during post-production operations also need to be tuned to minimize reductions in fly quality. Hypoxia, irradiation and vibration are important stressors that are experienced by pupae during production, packing and transportation. However, little is known about the impacts of such stressors on Q-fly quality. We conducted two laboratory experiments to investigate the response of Q-fly to such post-production stressors, with the aim of then developing guidelines that minimize quality reductions for SIT programs. In Experiment 1, Q-fly pupae of different ages (3, 6 or 9-day old) were exposed to vibrations for 5, 30, 60 and 300 s. Flight ability tests revealed that 6- and 9-day-old pupae were the most sensitive to vibration, with significant reduction in emergence and percentage of fliers. In Experiment 2, the effect of hypoxia, irradiation and vibration, in isolation or in combination, was evaluated on 9-day-old pupae. Irradiation and vibration exhibited a multiplicative negative effect on flight ability parameters. It is recommended not to subject pupae to intense or extensive vibration during the late pupal stage and to avoid prolonged periods of hypoxia.

不育昆虫技术（SIT）是一种良性害虫控制策略，目前正在进行实质性更新，以控制昆士兰果蝇（Q-fly），这是澳大利亚主要的园艺害虫。高质量果蝇的生产和交付对于有效的昆虫不育技术至关重要。尽管通常都在努力提高工厂水平（数量和质量）的产量，但还需要调整后期生产操作中的方法和条件，以最大程度地降低果蝇质量的下降。缺氧，辐射和振动是p在生产，包装和运输过程中所经历的重要压力源。但是，关于这种压力源对Q-fly质量的影响知之甚少。我们进行了两个实验室实验，以研究Q-fly对此类生产后应激源的反应，然后旨在制定准则，以最大程度地减少SIT计划的质量降低。在实验1中，将不同年龄（3、6或9天大）的Q-蝇to暴露于振动中5、30、60和300 s。飞行能力测试表明，6日龄和9日龄的p对振动最敏感，显着减少了飞行物和飞行物的百分比。在实验2中，对9天大的p进行了单独或组合的低氧，辐射和振动的影响评估。辐射和振动对飞行能力参数表现出成倍的负面影响。建议不要在p后期使p受到强烈或广泛的振动，并避免长时间缺氧。将不同年龄（3、6或9天大）的Q-蝇p暴露于振动5、30、60和300 s。飞行能力测试表明，6日龄和9日龄的p对振动最敏感，显着减少了飞行物和飞行物的百分比。在实验2中，对9天大的p进行了单独或组合的低氧，辐射和振动的影响评估。辐射和振动对飞行能力参数表现出成倍的负面影响。建议不要在p后期使p受到强烈或广泛的振动，并避免长时间缺氧。将不同年龄（3、6或9天大）的Q-蝇p暴露于振动5、30、60和300 s。飞行能力测试表明，6天和9天大的p对振动最敏感，飞羽的出现和飞行百分比的显着降低。在实验2中，对9天大的p进行了单独或组合的低氧，辐射和振动的效果评估。辐射和振动对飞行能力参数表现出成倍的负面影响。建议不要在p后期使p受到强烈或广泛的振动，并避免长时间缺氧。显着减少了飞行物和飞行物的百分比。在实验2中，对9天大的p进行了单独或组合的低氧，辐射和振动的影响评估。辐射和振动对飞行能力参数表现出成倍的负面影响。建议不要在p后期使p受到强烈或广泛的振动，并避免长时间缺氧。显着减少了飞行物和飞行物的百分比。在实验2中，对9天大的p进行了单独或组合的低氧，辐射和振动的影响评估。辐射和振动对飞行能力参数表现出成倍的负面影响。建议不要在p后期使p受到强烈或广泛的振动，并避免长时间缺氧。