Heat shock proteins (HSPs) and HSP70-HSP90 organizing proteins (HOPs) are related, and they function together to maintain cellular homeostasis and respond to stress. In the present study, we reported the first molecular characteristics of HSP70 (designated as CeHSP70) and HOP (designated as CeHOP) genes from the freshwater green algae Closterium ehrenbergii and examined the changes in their expression profiles under heat stress and toxic chemicals treatment. CeHSP70 presented the conserved motif patterns and EEVD domain specific to cytosolic HSP70; CeHOP contained a typical domain of TPR repeats. Real-time PCR analysis showed that thermal stress considerably up-regulated both CeHOP and CeHSP70. In addition, the genes were significantly induced by CuCl₂, CuSO₄, and NiSO₄, but not by K₂Cr₂O₇, herbicide, and endocrine disrupting chemicals. These results suggest that CeHOP and CeHSP70 function together and play a role in responses to specific stressors and indicate their possible use as sensitive specific biomarkers in risk assessments.

热休克蛋白 (HSP) 和 HSP70-HSP90 组织蛋白 (HOP) 相关，它们共同发挥作用以维持细胞稳态并对压力做出反应。在本研究中，我们首次报道了来自淡水绿藻新月藻 Ehrenbergii的HSP70 （命名为CeHSP70 ）和HOP （命名为CeHOP ）基因的分子特征，并检查了它们在热应激和有毒化学物质处理下表达谱的变化。 CeHSP70 呈现出胞质 HSP70 特有的保守基序模式和 EEVD 结构域； CeHOP 包含一个典型的 TPR 重复结构域。实时PCR分析表明，热应激显着上调了CeHOP和CeHSP70 。此外，这些基因被CuCl ₂ 、CuSO ₄和NiSO ₄显着诱导，但不被K ₂ Cr ₂ O ₇ 、除草剂和内分泌干扰化学品显着诱导。这些结果表明， CeHOP和CeHSP70共同发挥作用，并在对特定应激源的反应中发挥作用，并表明它们可能用作风险评估中敏感的特定生物标志物。