Cassava rhizome is an attractive agricultural residue from cassava-processing industry. With its lignocellulosic structure, cassava rhizome can be converted to simple sugars such as glucose, which is a precursor for succinic acid production. In this study, ozonation and alkaline pretreatments were chosen to enhance liberation of fermentable sugars from cassava rhizomes. Lignin removal and cellulose content were used as the criteria for the pretreatment performance. The maximum lignin removal of 56.47% ww⁻¹ was obtained from alkaline pretreatment under the condition using 2% NaOH at 120 °C and 30 min reaction time, while ozonation pretreatment for 60 min reaction time achieved 18.7% ww⁻¹ lignin removal. The extended reaction time for ozonation did not increase the lignin degradation while the cellulose content slightly increased. Higher concentration of NaOH resulted in decreases on the net weight of cassava rhizome after the pretreatment process. Fermentation of the enzymatic hydrolysate of alkaline-pretreated cassava rhizome led to the maximum succinic acid concentration (11.25 gL⁻¹) and glucose utilization (99.53% ww⁻¹). This study provided an alternative option to convert cassava rhizomes to biochemical products in biorefineries.

木薯根茎是木薯加工业的一种有吸引力的农业残留物。木薯根状茎具有木质纤维素的结构，可以转化为单糖，例如葡萄糖，它是琥珀酸生产的前体。在这项研究中，选择臭氧化和碱性预处理以增强木薯根茎中可发酵糖的释放。木质素去除和纤维素含量被用作预处理性能的标准。在120°C和30分钟的反应时间，使用2％NaOH的条件下进行碱预处理后，木质素的最大去除量为56.47％ww ^-1，而臭氧化预处理60分钟的反应时间则达到18.7％ww ^-1木质素去除。延长的臭氧化反应时间并没有增加木质素的降解，而纤维素含量却略有增加。预处理后，较高浓度的NaOH导致木薯根茎的净重降低。碱预处理的木薯根茎的酶水解产物的发酵导致最大的琥珀酸浓度（11.25 gL ^-1）和葡萄糖利用率（99.53％ww ^-1）。这项研究为将木薯根茎转化为生物精炼厂中的生化产品提供了另一种选择。