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Dietary lipid concentrations influence growth, body composition, morphology of the liver and mid-intestine, and antioxidant status of marbled eel ( Anguilla marmorata )
Aquaculture International ( IF 2.2 ) Pub Date : 2020-08-13 , DOI: 10.1007/s10499-020-00590-5
Shi-Jun Chen , Peng Liu , You-Ming Jia , Hong-Ping Liao , Shao-Xuan Zhu , Li-Liu Zhou , Xue-Ming Dan , Li Liu , Jia-Hao Li , Su-Bi Zheng , Jun-Jiang Yang , Lian Gan

An 8-week feeding trial was conducted to explore the effect of dietary lipid concentrations on growth, body composition, morphology of liver, and mid-intestine and antioxidant status in marbled eel (Anguilla marmorata). Fish (initial average weight 6.86 ± 0.11 g) were held in 18 tanks (inside diameter 80 cm, height 60 cm, volume 300 L, 25 fish/tank), with triplicate treatment groups being fed to apparent satiation twice each day on isonitrogenous (421 g/kg crude protein) diets containing different concentrations of lipid (41.2, 73.9, 113.9, 146.2, 178.1, and 216.7 g/kg). The sampling procedure was implemented after 24 h of fasting. Fish fed the diets with 178.1 and 216.7 g lipid/kg grew better than fish fed the other diets. The lowest feed intake and the highest feed conversion rate were found in 41.2 g/kg group. Muscle lipid concentrations and heights of intestinal folds, enterocytes, and microvilli in mid-intestine increased as dietary lipid concentrations increased from 41.2 to 178.1 g/kg. Hepatocyte hypertrophy and lipid vacuolization were seen in liver of the fish fed 216.7 g/kg diet. Based on broken-line analysis of serum total antioxidant capacity and quadratic regression analysis of serum glutathione peroxidase and catalase, it was concluded that dietary lipid concentrations of 125.5–159.2 g/kg promoted antioxidant status. A broken-line analysis of data for specific growth rate and weight gain gave an estimated optimal dietary lipid concentration of 195.3–198.7 g/kg. Marbled eel had quite a high demand for dietary lipid to support good growth, but excessive lipid level (216.7 g/kg) may have an adverse impact on liver structure. Moreover, marbled eel may not tolerate high concentration of dietary carbohydrate.



中文翻译:

饮食中的脂类浓度会影响生长,身体组成,肝脏和中肠的形态以及大理石纹鳗的抗氧化状态

8周饲养试验以探索对增长,身体组成,肝脏的形态,和中肠和抗氧化状态在大理石鳗鱼膳食脂质浓度的效果(安圭拉marmorata)。将鱼(初始平均重量6.86±0.11 g)放在18个水箱中(内径80厘米,高60厘米,容积300升,鱼/罐25条),一式三份的治疗组每天两次在异氮( 421 g / kg粗蛋白)日粮中含有不同浓度的脂质(41.2、73.9、113.9、146.2、178.1和216.7 g / kg)。禁食24小时后实施采样程序。饲喂178.1 g / kg和216.7 g脂质/ kg日粮的鱼比饲喂其他日粮的鱼生长更好。41.2 g / kg组的最低采食量和最高饲料转化率。随着饮食中脂质浓度从41.2 g / kg增加到178.1 g / kg,肠道中部肌肉的脂质浓度和肠皱褶,肠上皮细胞和微绒毛的高度增加。饲喂216.7 g / kg日粮的鱼肝中可见肝细胞肥大和脂质空泡化。根据血清总抗氧化剂能力的折线分析和血清谷胱甘肽过氧化物酶和过氧化氢酶的二次回归分析,得出的结论是日粮脂质浓度为125.5–159.2 g / kg可以促进抗氧化状态。对特定生长率和体重增加的数据进行的折线分析得出最佳饮食脂质浓度估计为195.3–198.7 g / kg。鳗鱼对膳食脂质的需求很高,以支持其良好的生长,但是过多的脂质水平(216.7 g / kg)可能会对肝脏结构产生不利影响。此外,大理石鳗可能无法忍受高浓度的饮食碳水化合物。根据血清总抗氧化剂能力的折线分析和血清谷胱甘肽过氧化物酶和过氧化氢酶的二次回归分析,得出的结论是日粮脂质浓度为125.5–159.2 g / kg可以促进抗氧化状态。对特定生长率和体重增加的数据进行的折线分析得出最佳饮食脂质浓度估计为195.3–198.7 g / kg。鳗鱼对膳食脂质的需求很高,以支持其良好的生长,但是过多的脂质水平(216.7 g / kg)可能会对肝脏结构产生不利影响。此外,大理石鳗可能无法忍受高浓度的饮食碳水化合物。根据血清总抗氧化剂能力的折线分析和血清谷胱甘肽过氧化物酶和过氧化氢酶的二次回归分析,得出的结论是日粮脂质浓度为125.5–159.2 g / kg可以促进抗氧化状态。对特定生长率和体重增加的数据进行的折线分析得出最佳饮食脂质浓度估计为195.3–198.7 g / kg。鳗鱼对膳食脂质的需求很高,以支持其良好的生长,但是过多的脂质水平(216.7 g / kg)可能会对肝脏结构产生不利影响。此外,大理石鳗可能无法忍受高浓度的饮食碳水化合物。对特定生长率和体重增加的数据进行的折线分析得出最佳饮食脂质浓度估计为195.3–198.7 g / kg。鳗鱼对膳食脂质的需求很高,以支持其良好的生长,但是过多的脂质水平(216.7 g / kg)可能会对肝脏结构产生不利影响。此外,大理石鳗可能无法忍受高浓度的饮食碳水化合物。对特定生长率和体重增加的数据进行的折线分析得出最佳饮食脂质浓度估计为195.3–198.7 g / kg。鳗鱼对膳食脂质的需求很高,以支持其良好的生长,但是过多的脂质水平(216.7 g / kg)可能会对肝脏结构产生不利影响。此外,大理石鳗可能无法忍受高浓度的饮食碳水化合物。

更新日期:2020-08-14
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