Alkaline phosphatase (ALP) is important in the diagnosis of many diseases. Because ALP is used to detect biomarkers for many diseases, many researchers conduct investigations to develop ALP detection strategies. The use of fluorescent material has attracted attention because of the technique's high sensitivity and the low sample volume required. Herein, we review and discuss the working mechanisms and advantages of four main categories:DNA fluorescent probes, molecular fluorescent probes, chemical coordination‐based probes, and nanoparticle probes. Development prospects and trends are also discussed.

中文翻译：

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荧光材料在碱性磷酸酶活性检测中的应用

碱性磷酸酶 (ALP) 在许多疾病的诊断中都很重要。由于 ALP 用于检测许多疾病的生物标志物，许多研究人员进行调查以开发 ALP 检测策略。由于该技术的高灵敏度和所需的样品量少，荧光材料的使用引起了人们的关注。在此，我们回顾和讨论了四大类的工作机制和优势：DNA荧光探针、分子荧光探针、化学配位探针和纳米粒子探针。还讨论了发展前景和趋势。