Abstract

Oleaginous filamentous fungi grown under the nitrogen limitation, accumulate high amounts of lipids in the form of triacylglycerides (TAGs) with fatty acid profiles similar to plant and fish oils. In this study, we investigate the effect of six phosphorus source concentrations combined with two types of nitrogen substrate (yeast extract and ammonium sulphate), on the biomass formation, lipid production, and fatty acid profile for nine oleaginous Mucoromycota fungi. The analysis of fatty acid profiles was performed by gas chromatography with flame ionization detector (GC-FID) and the lipid yield was estimated gravimetrically. Yeast extract could be used as both nitrogen and phosphorus source, without additional inorganic phosphorus supplementation. The use of inorganic nitrogen source (ammonium sulphate) requires strain-specific optimization of phosphorus source amount to obtain optimal lipid production regarding quantity and fatty acid profiles. Lipid production was decreased in ammonium sulphate-based media when phosphorus source was limited in all strains except for Rhizopus stolonifer. High phosphorus source concentration inhibited the growth of Mortierella fungi. The biomass (22 g/L) and lipid (14 g/L) yield of Umbelopsis vinacea was the highest among all the tested strains.

Key points

• The strain specific P requirements of Mucoromycota depend on the nature of N source.

• Yeast extract leads to consistent biomass and lipid yield and fatty acids profiles.

• Umbelopsis vinacea showed the highest biomass (22 g/L) and lipid (14 g/L) yield.

• High P source amounts inhibit the growth of Mortierella fungi.

中文翻译：

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磷源和氮基质性质对油性毛霉菌真菌生物量生产和脂质积累的影响。

摘要

在氮限制下生长的油生丝状真菌以甘油三酸酯（TAGs）的形式积累了大量的脂质，其脂肪酸特征类似于植物油和鱼油。在这项研究中，我们调查了六个磷源浓度与两种类型的氮底物（酵母提取物和硫酸铵）的组合对九种含油性毛霉菌的生物量形成，脂质产生和脂肪酸谱的影响。菌类。通过具有火焰离子化检测器（GC-FID）的气相色谱法进行脂肪酸谱分析，并通过重量分析法估算脂质产率。酵母提取物可以用作氮和磷的来源，而无需额外添加无机磷。使用无机氮源（硫酸铵）需要对磷源的量进行特定于菌株的优化，以获得有关数量和脂肪酸分布的最佳脂质生产。当除茎根霉外的所有菌株中的磷源都受到限制时，基于硫酸铵的培养基中的脂质产生降低。高磷源浓度抑制了莫氏菌的生长。的生物量（22 g / L）和脂质（14 g / L）的产量在所有测试的菌株中，伞形葡萄菌最高。

关键点

•毛霉菌菌株对磷的特定需求取决于氮源的性质。

•酵母提取物可产生一致的生物量，脂质产量和脂肪酸谱。

•伞形葡萄菌显示出最高的生物量（22 g / L）和脂质（14 g / L）产量。

•高磷源会抑制羊肚菌的生长。