ABSTRACT

The study was aimed to investigate the effect of alpha-lipoic acid (ALA) on human umbilical vein endothelial cells (HUVECs) injury induced by hydrogen peroxide (H₂O₂) and to explore its possible mechanisms. We established the H₂O₂-induced HUVECs injury model and the ALA treatment groups in which HUVECs were co-incubated with H₂O₂ (250 μmol/L) and different final concentrations of ALA (100,200,400 μmol/L) for 48 h. Cell survival rate assay and LDH activity assay were carried out. The levels of related proteins were performed by Western Blot. We observed that H₂O₂ administration resulted in an increase in the LDH activity and a decrease in cell survival rate. The expression levels of Nox4, Bax, NF-κB p65, Caspase-9, Caspase-3, iNOS, VCAM-1 and ICAM-1 were up-regulated, while the expression level of Bcl-2 was down-regulated. All these factors were significantly improved by ALA treatment. In brief, ALA treatment ameliorates H₂O₂-induced HUVECs damage by inhibiting inflammation and oxidative stress.

摘要

该研究旨在研究α-硫辛酸（ALA）对过氧化氢（H ₂ O ₂）诱导的人脐静脉内皮细胞（HUVEC）损伤的作用，并探讨其可能的机制。我们建立了H ₂ O ₂诱导的HUVEC损伤模型和ALA治疗组，其中HUVEC与H ₂ O ₂（250μmol/ L）和不同终浓度的ALA（100,200,400μmol/ L）共同孵育48 h 。进行细胞存活率测定和LDH活性测定。相关蛋白质的水平通过蛋白质印迹法进行。我们观察到H ₂ O ₂施用导致LDH活性增加和细胞存活率降低。Nox4，Bax，NF-κBp65，Caspase-9，Caspase-3，iNOS，VCAM-1和ICAM-1的表达水平上调，而Bcl-2的表达水平下调。所有这些因素均通过ALA治疗得到了明显改善。简而言之，ALA处理通过抑制炎症和氧化应激改善了H ₂ O ₂诱导的HUVEC损伤。