Engineering Isoprenoid Quinone Production in Yeast.,ACS Synthetic Biology

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Engineering Isoprenoid Quinone Production in Yeast.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-08-12 , DOI: 10.1021/acssynbio.0c00081
Divjot Kaur ₁ , Duha Alkhder ₂ , Christophe Corre ₁ , Fabrizio Alberti ₁

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Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also produced by all domains of life. Here, we report the engineering of a baker’s yeast strain, Saccharomyces cerevisiae EPYFA3, for the production of isoprenoid quinones. Our yeast strain was developed through overexpression of the shikimate pathway in a well-established recipient strain (S. cerevisiae EPY300) where the mevalonate pathway is overexpressed. As a proof of concept, our new host strain was used to overproduce the endogenous isoprenoid quinone coenzyme Q₆, resulting in a nearly 3-fold production increase. EPYFA3 represents a valuable platform for the heterologous production of high value isoprenoid quinones. EPYFA3 will also facilitate the elucidation of isoprenoid quinone biosynthetic pathways.

中文翻译：

在酵母中工程化异戊二烯醌生产。

类异戊二烯醌是生物活性分子，包括类异戊二烯链和醌头。传统上发现它们参与初级代谢，在那里它们充当电子转运蛋白，但特殊的类异戊二烯醌也由生命的所有领域产生。在这里，我们报告了用于生产类异戊二烯醌的面包酵母菌株Saccharomyces cerevisiae EPYFA3的工程。我们的酵母菌株是通过在一个成熟的受体菌株 ( S. cerevisiae EPY300) 中过表达莽草酸途径而开发的，其中甲羟戊酸途径过表达。作为概念证明，我们的新宿主菌株用于过度生产内源性类异戊二烯醌辅酶 Q ₆，导致产量增加近 3 倍。EPYFA3 代表了异源生产高价值类异戊二烯醌的宝贵平台。EPYFA3 还将促进类异戊二烯醌生物合成途径的阐明。

更新日期：2020-09-20

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