The genus Enterobacter comprises common pathogens and has a complicated taxonomy. Precise taxonomic assignation lays a foundation for microbiology. In this study, we updated the Enterobacter taxonomy based on robust genome analyses. We found that all Enterobacter subspecies assignments were incorrect. Enterobacter cloacae subsp. dissolvens and Enterobacter hormaechei subsp. hoffmannii are species (Enterobacter dissolvens and Enterobacter hoffmannii, respectively) rather than subspecies. Enterobacter xiangfangensis, Enterobacter hormaechei subsp. oharae, and Enterobacter hormaechei subsp. steigerwaltii are not Enterobacter hormaechei subspecies but belong to the same species (Enterobacter xiangfangensis). Enterobacter timonensis should be removed to Pseudenterobacter, a novel genus. We then reported two novel species, Enterobacter quasiroggenkampii and Enterobacter quasimori, by genome- and phenotype-based characterization. We also applied the updated taxonomy to curate 1,997 Enterobacter genomes in GenBank. Species identification was changed following our updated taxonomy for the majority of publicly available strains (1,542, 77.2%). The most common Enterobacter species was E. xiangfangensis. We identified 14 novel tentative Enterobacter genomospecies. This study highlights that updated and curated taxonomic assignments are the premise of correct identification.

中文翻译：

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肠杆菌的精确物种鉴定：基于基因组序列的研究，报道了两个新物种，quasrogroggenkampii sp.。十一月 和准肠杆菌。十一月

属肠杆菌包括常见病原体和具有复杂的分类。精确的分类分配为微生物学奠定了基础。在这项研究中，我们基于可靠的基因组分析更新了肠杆菌分类法。我们发现所有肠杆菌亚种分配均不正确。阴沟肠杆菌亚种。dissolvens和肠杆菌霍氏亚种。霍夫曼氏菌是物种（分别为溶肠杆菌和霍夫曼氏杆菌）而不是亚种。香肠杆菌，霍马肠杆菌亚种。oharae和霍曼肠杆菌亚种。steigerwaltii不是霍马氏肠杆菌亚种，但属于同一物种（香坊肠杆菌）。timonensis肠杆菌应移至Pseudenterobacter（一种新属）中。然后，我们通过基于基因组和表型的特征报告了两个新物种，拟杆菌肠杆菌和拟杆菌肠杆菌。我们还应用了更新的分类法来整理1,997肠杆菌GenBank中的基因组。在我们更新了大多数公开可用菌株的分类之后，物种鉴定发生了变化（1,542，77.2％）。最常见的肠杆菌是香肠杆菌。我们确定了14个新的暂定肠杆菌基因型。这项研究强调，更新和精心策划的分类分配是正确识别的前提。