This study aimed to evaluate the polyphenolic composition along with the biological activity of guabiroba (Campomanesia xanthocarpa Berg.) fruits using comprehensive two‐dimensional liquid chromatography (LC × LC). A simplex centroid design comprising three solvents (methanol, 2% acetic acid, and acetonitrile) was used to optimize the extraction mixture for polyphenols from ripe and unripe guabiroba fruits. A quantitative LC × LC platform was proposed to characterize the guabiroba extracts using a RP‐Amide column and a C18 column in the first and second dimensions, respectively. Antidiabetic properties, using in vitro enzyme assay models and in vivo antioxidant activity with the eukaryote model Saccharomyces cerevisiae, was measured. Total phenolics compounds were more efficiently extracted with 2% acetic acid solution and acetonitrile (50:50, v/v). A total of 37 different compounds were identified and quantified using the proposed LC × LC method (linearity ranging from 0.9990 to 0.9994, intra‐ and interday precision from 0.40 to 10.57% and, accuracy from 81.89 to 108.98%). Significant differences were observed between ripe and unripe guabiroba fruits, especially for the compounds geraldone and methyl galangin isomer. Guabiroba fruits showed significant antidiabetic and antioxidant properties and may be potentially adopted as part of dietary strategies in the management of early stages of type 2 diabetes and associated complications.

中文翻译：

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综合二维液相色谱法测定番石榴果实中具有生物活性的多酚类化合物（Campomanesia xanthocarpa Berg。）。

这项研究旨在使用综合二维液相色谱（LC×LC）来评估瓜比罗巴（Camponesnes xanthocarpa Berg。）水果的多酚成分以及其生物活性。使用包含三种溶剂（甲醇，2％乙酸和乙腈）的单纯形质心设计来优化从成熟和未成熟的瓜比罗巴水果中提取多酚的提取混合物。提出了定量LC×LC平台以分别使用RP-Amide色谱柱和C18色谱柱在第一维和第二维上表征瓜比罗巴提取物的特性。使用体外酶测定模型和真核生物模型酿酒酵母（Saccharomyces cerevisiae）的体内抗氧化活性，进行了测量。用2％的乙酸溶液和乙腈（50:50，v / v）更有效地萃取总酚类化合物。使用提议的LC×LC方法鉴定和定量了37种不同的化合物（线性范围为0.9990至0.9994，日内和日间精度为0.40至10.57％，准确度为81.89至108.98％）。已观察到成熟和未成熟的瓜比罗巴水果之间存在显着差异，尤其是对于化合物杰拉酮和甲基高良姜素异构体而言。番石榴果实显示出显着的抗糖尿病和抗氧化特性，可能在管理2型糖尿病及相关并发症的早期阶段中被作为饮食策略的一部分。