The Insulin and Insulin-like Signaling (IIS) network regulates cellular processes including pre- and post-natal growth, cellular development, wound healing, reproduction, and longevity. Despite their importance on the physiology of vertebrates, the study of the specific functions of the top regulators of the IIS network - insulin-like growth factors (IGFs) and IGF binding proteins (IGFBPs) has been mostly limited to a few model organisms. To expand our understanding of this network, we performed quantitative gene expression of IGF hormones in liver and qualitative expression of IGFBPs across tissues and developmental stages in a model reptile, the brown anole lizard (Anolis sagrei). We found that lizards express IGF2 across all life stages (pre-oviposition embryos to adulthood), and at a higher level than IGF1, which is opposite to patterns seen in lab rodents but similar to those seen in humans and other vertebrate models. IGFBP expression was ubiquitous across tissues (brain, gonad, heart, liver, skeletal muscle, tail, and regenerating tail) in adults, apart from IGFBP5 which was variable. These findings provide an essential foundation for further developing the anole lizard as a physiological and biomedical reptile model, as well as expanding our understanding of the function of the IIS network across species.

中文翻译：

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模型爬行动物中 IGF 激素和 IGF 结合蛋白随时间和组织的基因表达。


胰岛素和胰岛素样信号传导 (IIS) 网络调节细胞过程，包括产前和产后生长、细胞发育、伤口愈合、繁殖和寿命。尽管对脊椎动物的生理学很重要，但对 IIS 网络顶级调节因子 - 胰岛素样生长因子 (IGF) 和 IGF 结合蛋白 (IGFBP) 的特定功能的研究大多仅限于少数模型生物。为了扩大我们对这个网络的理解，我们在模型爬行动物棕色变色龙（Anolis sagrei）中进行了肝脏中 IGF 激素的定量基因表达以及跨组织和发育阶段的 IGFBP 的定性表达。我们发现蜥蜴在所有生命阶段（产卵前胚胎到成年）都表达 IGF2，并且表达水平高于 IGF1，这与实验室啮齿动物中观察到的模式相反，但与人类和其他脊椎动物模型中观察到的模式相似。 IGFBP 表达在成人的组织（脑、性腺、心脏、肝脏、骨骼肌、尾巴和再生尾巴）中普遍存在，但 IGFBP5 的表达是可变的。这些发现为进一步开发变色蜥蜴作为生理和生物医学爬行动物模型以及扩大我们对跨物种 IIS 网络功能的理解提供了重要基础。