We report two novel RNA viruses from yellow crazy ants, (Anoplolepis gracilipes) detected using next-generation sequencing. The complete genome sequences of the two viruses were 10,662 and 8,238 nucleotides in length, respectively, with both possessing two open reading frames with three conserved protein domains. The genome organization is characteristic of members of the genus Triatovirus in the family Dicistroviridae. The two novel viruses were tentatively named "Anoplolepis gracilipes virus 1" and "Anoplolepis gracilipes virus 2" (AgrV-1 and AgrV-2). Phylogenetic analyses based on amino acid sequences of the non-structural polyprotein (ORF1) suggest that the two viruses are triatovirus-like viruses. This is the first report on the discovery of novel triatovirus-like viruses in yellow crazy ants with a description of their genome structure (two ORFs and conserved domains of RNA helicase, RNA-dependent RNA polymerase, and capsid protein), complete sequences, and viral prevalence across the Asia-Pacific region.

中文翻译：

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在黄色的疯狂蚂蚁（Anoplolepis gracilipes）中检测到的两种新型双顺反病毒的完整基因组序列。

我们报告了使用新一代测序技术从黄色疯狂蚂蚁（Anoplolepis gracilipes）发现的两种新型RNA病毒。两种病毒的完整基因组序列长度分别为10662和8238个核苷酸，均具有两个带有三个保守蛋白结构域的开放阅读框。基因组的组织是Dicistroviridae家族中Triatovirus属成员的特征。暂时将这两种新型病毒分别命名为“细线虫无病毒1”和“细线虫无病毒2”（AgrV-1和AgrV-2）。基于非结构性多蛋白（ORF1）氨基酸序列的系统进化分析表明，这两种病毒是三角病毒样病毒。