Combining organometallics and biology has generated broad interest from scientists working on applications from in situ drug release to biocatalysis. Engineered enzymes and biohybrid catalysts (also referred to as artificial enzymes) have introduced a wide range of abiotic chemistry into biocatalysis. Predominantly, this work has concentrated on using these catalysts for single step in vitro reactions. However, the promise of using these hybrid catalysts in vivo and combining them with synthetic biology and metabolic engineering is vast. This report will briefly review recent advances in artificial metalloenzyme design, followed by summarising recent studies that have looked at the use of these hybrid catalysts in vivo and in enzymatic cascades, therefore exploring their potential for synthetic biology.

中文翻译：

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用于合成生物学的设计师金属酶：用于催化的酶杂化物。

有机金属和生物学的结合引起了从事从原位药物释放到生物催化应用的科学家们的广泛兴趣。工程酶和生物混合催化剂（也称为人工酶）已将广泛的非生物化学引入生物催化。这项工作主要集中在将这些催化剂用于单步体外反应。然而，在体内使用这些混合催化剂并将它们与合成生物学和代谢工程相结合的前景是巨大的。本报告将简要回顾人工金属酶设计的最新进展，然后总结最近的研究，这些研究着眼于这些混合催化剂在体内和酶促级联中的使用，从而探索它们在合成生物学中的潜力。