Major developmental processes such as gastrulation and early embryogenesis rely on a complex network of cell-cell interactions, chromatin remodeling, and transcriptional regulators. This makes it challenging to study early development when using bulk populations of cells. Recent advances in single-cell technologies have allowed researchers to better understand the interactions between different molecular modalities and the heterogeneities within classically defined cell types. As new single-cell technologies mature, they have the potential of providing a step-change in our understanding of embryogenesis. In this review, we summarize recent advances in single-cell technologies with particular focus on those that lend insight into early organogenesis. We then discuss current pitfalls and implications for future research.

中文翻译：

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单细胞基因组学和发育生物学：超越细胞类型目录的生成。

原肠胚形成和早期胚胎发生等主要发育过程依赖于细胞间相互作用、染色质重塑和转录调节因子的复杂网络。这使得在使用大量细胞时研究早期发育具有挑战性。单细胞技术的最新进展使研究人员能够更好地了解不同分子模式之间的相互作用以及经典定义的细胞类型内的异质性。随着新的单细胞技术的成熟，它们有可能使我们对胚胎发生的理解发生根本性的变化。在这篇综述中，我们总结了单细胞技术的最新进展，特别关注那些有助于深入了解早期器官发生的技术。然后我们讨论当前的陷阱和对未来研究的影响。