Vibrio parahaemolyticus and Escherichia coli are two major foodborne pathogens. In this paper, the antibiofilm activities of the ethanol extract of cinnamon against these two bacteria were studied in detail . The antibacterial activity and the MIC of the extract were determined, and the inhibition and removing effects of the extract on the biofilms of V. parahaemolyticus and E. coli were investigated. The biofilms stained with fluorescein isothiocyanate (FITC) and concanavalin A (Con A) were also observed by confocal laser scanning microscope (CLSM). The results indicated that the extract exhibited high antibacterial activity, with the MIC against V. parahaemolyticus and E. coli was 6.25 mg/mL. The effects on V. parahaemolyticus biofilm were significant with the inhibition rate of 75.46% at MIC, and the eradication rate of 93.26% at 32MIC, respectively. As to E. coli , the inhibition rate was 48.18% at MIC, and the eradication rate was 46.16% at 8MIC. Meanwhile, the extract could notably reduce the metabolic activities and the secretion of EPS in biofilm, it inhibited 78.57% EPS formation in V. parahaemolyticus biofilm at MIC, and eliminated 61.28% EPS in mature biofilm at 4MIC. CLSM images showed that the EPS of the treated biofilm became thinner and biofilm structure was looser, when compared with the untreated control. This study elucidated that the cinnamon extract was effective to prevent biofilm formation and eradicate mature biofilms of V. parahaemolyticus and E. coli .

中文翻译：

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肉桂提取物对副溶血性弧菌和大肠杆菌的抗生物膜活性

副溶血性弧菌和大肠杆菌是两种主要的食源性病原体。本文详细研究了肉桂乙醇提取物对这两种细菌的​​抗生物膜活性。测定了提取物的抗菌活性和MIC，考察了提取物对副溶血性弧菌和大肠杆菌生物膜的抑制和去除作用。还通过共聚焦激光扫描显微镜 (CLSM) 观察了用异硫氰酸荧光素 (FITC) 和刀豆蛋白 A (Con A) 染色的生物膜。结果表明，该提取物具有较高的抗菌活性，对副溶血性弧菌和大肠杆菌的 MIC 为 6.25 mg/mL。对副溶血性弧菌生物膜作用显着，MIC抑制率为75.46%，32MIC根除率为93.26%，分别。对于大肠杆菌，MIC抑制率为48.18%，8MIC根除率为46.16%。同时，该提取物能显着降低生物膜中EPS的代谢活性和分泌，在MIC下抑制副溶血性弧菌生物膜中78.57%的EPS形成，4MIC下消除成熟生物膜中61.28%的EPS。CLSM 图像显示，与未处理的对照相比，处理后的生物膜的 EPS 变薄，生物膜结构更松散。该研究阐明了肉桂提取物可有效防止生物膜形成并根除副溶血性弧菌和大肠杆菌的成熟生物膜。该提取物可显着降低生物膜中EPS的代谢活性和分泌，在MIC时抑制副溶血性弧菌生物膜中78.57%的EPS形成，4MIC时消除成熟生物膜中61.28%的EPS。CLSM 图像显示，与未处理的对照相比，处理后的生物膜的 EPS 变薄，生物膜结构更松散。该研究阐明了肉桂提取物可有效防止生物膜形成并根除副溶血性弧菌和大肠杆菌的成熟生物膜。该提取物可显着降低生物膜中EPS的代谢活性和分泌，在MIC时抑制副溶血性弧菌生物膜中78.57%的EPS形成，4MIC时消除成熟生物膜中61.28%的EPS。CLSM 图像显示，与未处理的对照相比，处理后的生物膜的 EPS 变薄，生物膜结构更松散。该研究阐明了肉桂提取物可有效防止生物膜形成并根除副溶血性弧菌和大肠杆菌的成熟生物膜。