Lipid composition of liposomal bilayer should alter the cell response for permeability, transport, and uptake in small intestine. This work was done to investigate the transport and uptake of liposomes composed of docosahexaenoic acid‐enriched phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and sulfoquinovosyl diacylglycerol (SQDG) derived from marine products on multilamellar vesicles (MLV) in small intestinal epithelial cell models. The results showed that addition of PtdSer and SQDG as liposomal bilayer could improve the efficiency entrapment of liposomes. The liposomes containing PtdSer showed higher transport and uptake through both Caco‐2 cell and M cell monolayers as compared to PtdCho‐MLV. SQDG‐containing liposomes exhibited only higher transport through M cell monolayer, while its uptake effect was higher both in Caco‐2 cell and M cell monolayers. The results of experiments done with endocytosis inhibitors indicated that PtdCho‐MLV must be transported via macropinocytosis and uptaken by phagocytosis in M cell monolayer model. PtdCho/PtdSer‐MLV and PtdCho/SQDG‐MLV might be transported and uptaken through M cell monolayer by phagocytosis. The result also indicated that PtdCho/SQDG‐MLV could open the tight junction of small intestinal epithelial cell monolayers. Furthermore, our findings demonstrated that the surface status of cholesterol‐containing liposomes were smooth, but they did not affect their transport and uptake through Caco‐2 cell and M cell monolayers.

中文翻译：

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脂质体膜的脂质组成很大程度上影响其通过小肠上皮细胞模型的运输和摄取。


脂质体双层的脂质组成应改变细胞对小肠的渗透性、运输和摄取的反应。这项工作的目的是研究小肠上皮细胞模型中由富含二十二碳六烯酸的磷脂酰胆碱（PtdCho）、磷脂酰丝氨酸（PtdSer）和磺基喹诺酰二酰甘油（SQDG）组成的脂质体在小肠上皮细胞模型中的多层囊泡（MLV）上的转运和摄取。结果表明，添加PtdSer和SQDG作为脂质体双层可以提高脂质体的包封效率。与 PtdCho-MLV 相比，含有 PtdSer 的脂质体在 Caco-2 细胞和 M 细胞单层中表现出更高的转运和摄取能力。含SQDG的脂质体仅表现出较高的通过M细胞单层的转运，而其在Caco-2细胞和M细胞单层中的摄取效果均较高。使用内吞作用抑制剂进行的实验结果表明，PtdCho-MLV 必须通过巨胞饮作用转运，并在 M 细胞单层模型中通过吞噬作用摄取。 PtdCho/PtdSer-MLV 和 PtdCho/SQDG-MLV 可能通过吞噬作用通过 M 细胞单层转运和摄取。结果还表明，PtdCho/SQDG-MLV 可以打开小肠上皮细胞单层的紧密连接。此外，我们的研究结果表明，含胆固醇脂质体的表面状态是光滑的，但它们并不影响其通过Caco-2细胞和M细胞单层的运输和摄取。