In this work, we demonstrate a single‐view field filter (SVFF) device for the efficient filtration and enumeration of rare tumor cells in the blood. In our device, the track‐etched membrane is integrated within a low‐cost polymer‐film microfluidic chip, and multiplex microfiltration chambers are designed. Our device permits the performing of multiple sample tests on a single membrane and the dynamical observation of the entire filtration process in a single field of view. To characterize the device performance, our device is first tested using tumor cells, and three different cell behaviors are observed during the filtration process. Finally, we successfully apply our device for the separation of rare tumor cells from the lysed blood samples at various flow rates. The recovery rates of 93.3, 87.6, and 84.1% can be respectively achieved at the throughputs of 50, 100, and 150 μL/min. Our single‐view field filter (SVFF) device offers the advantages of label‐free filtration, efficient enumeration, easy integration, and low cost, and holds the potential to be used as an efficient tool for the filtration and enumeration of rare cells.

中文翻译：

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用于稀有肿瘤细胞过滤和计数的单视场过滤器设备。

在这项工作中，我们演示了一种单视场过滤器（SVFF）设备，可有效过滤和计数血液中的稀有肿瘤细胞。在我们的设备中，轨迹蚀刻膜集成在低成本的聚合物膜微流控芯片中，并设计了多重微滤室。我们的设备允许在单个膜上执行多个样品测试，并在单个视野中动态观察整个过滤过程。为了表征设备的性能，我们的设备首先使用肿瘤细胞进行了测试，并且在过滤过程中观察到了三种不同的细胞行为。最后，我们成功地将我们的设备应用于以各种流速从裂解血样中分离稀有肿瘤细胞。回收率分别为93.3、87.6和84。在50、100和150μL/ min的通量下可以分别达到1％。我们的单视场过滤器（SVFF）设备具有无标签过滤，有效枚举，易于集成和低成本的优点，并具有用作稀有细胞过滤和枚举的有效工具的潜力。