This work resulted in the development of a method based on fluorescence spectroscopy to differentiate between three corn varieties, standard, mutant and sweet, and to characterize the corn variety present in finished products. This was achieved by recording fluorescence emission spectra as a function of excitation wavelength. For a standard, non-transgenic and non-sweet corn, the maximum of the first peak is around 412–414 nm at the excitation wavelength equal to 280 nm and shifts to the longer emission wavelengths as the excitation wavelength increases. Also, the second peak is located at 535 nm or is slightly higher (537 to 540 nm) and does not vary for excitation wavelengths from 280 to 360 nm. For mutant corn, the position of the first peak is located at 420 nm and above for λ_ex = 280 nm, while the second peak starts at 525–530 nm (depending on the mutant) and never reaches 535 nm. Finally, for a sweet corn, the position of the first fluorescence emission peak is around 430 nm. If the sweet corn is non-hybrid, the position of the second emission peak is at 535 nm. A hybrid sweet corn has its second peak around 530 nm. Thus, fluorescence emission at 530 is characteristic of corn that has undergone natural or artificial genetic transformation. Finally, we found simple mathematical equations to calculate the percentage of amylopectin and amylose in a given corn.

中文翻译：

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标准玉米、突变玉米和甜玉米的荧光表征。

这项工作导致开发了一种基于荧光光谱的方法来区分标准、突变和甜玉米三个品种，并表征成品中存在的玉米品种。这是通过将荧光发射光谱记录为激发波长的函数来实现的。对于标准的非转基因和非甜玉米，在等于 280 nm 的激发波长处，第一个峰的最大值约为 412-414 nm，并且随着激发波长的增加而移动到更长的发射波长。此外，第二个峰位于 535 nm 或稍高（537 至 540 nm），并且对于 280 至 360 nm 的激发波长没有变化。对于突变玉米，第一个峰的位置位于 420 nm 及以上的 λ _ex = 280 nm，而第二个峰从 525–530 nm 开始（取决于突变体）并且从未达到 535 nm。最后，对于甜玉米，第一个荧光发射峰的位置在430 nm附近。如果甜玉米是非杂交的，第二个发射峰的位置在535 nm。杂交甜玉米的第二个峰在 530 nm 左右。因此，530 处的荧光发射是经过自然或人工遗传转化的玉米的特征。最后，我们找到了简单的数学方程来计算给定玉米中支链淀粉和直链淀粉的百分比。