Rhizoctonia solani is a widely distributed soilborne plant pathogen, and can cause significant economic losses to crop production. In chemical controls, SYP-14288 is highly effective against plant pathogens, including R. solani. To examine the sensitivity to SYP-14288, 112 R. solani isolates were collected from infected rice plants. An established baseline sensitivity showed that values of effective concentration for 50% growth inhibition (EC₅₀) ranged from 0.0003 to 0.0138 μg/ml, with an average of 0.0055 ± 0.0030 μg/ml. The frequency distribution of the EC₅₀ was unimodal and the range of variation factor (the ratio of maximal over minimal EC₅₀) was 46.03, indicating that all wild-type strains were sensitive to SYP-14288. To examine the risk of fungicide resistance, 20 SYP-14288-resistant mutants were generated on agar plates amended with SYP-14288. Eighteen mutants remained resistant after 10 transfers, and their fitness was significantly different from the parental strain. All of the mutants grew more slowly but showed high virulence to rice plants, though lower than the parental strain. A cross-resistance assay demonstrated that there was a positive correlation between SYP-14288 and fungicides having or not having the same mode of action with SYP-14288, including fluazinam, fentin chloride, fludioxonil, difenoconazole, cyazofamid, chlorothalonil, and 2,4-dinitrophen. This result showed a multidrug resistance induced by SYP-14288, which could be a concern in increasing the spectrum of resistance in R. solani to commonly used fungicides.

茄红枯菌是一种广泛传播的土壤传播植物病原体，可能对作物生产造成重大经济损失。在化学控制中，SYP-14288对植物病原体（包括茄红梭菌）非常有效。为了检查对SYP-14288的敏感性，从受感染的水稻植物中收集了112株solani solani分离株。建立的基线灵敏度表明，对50％生长抑制的有效浓度（EC ₅₀）值在0.0003至0.0138μg/ ml范围内，平均为0.0055±0.0030μg/ ml。EC ₅₀的频率分布是单峰的，并且变化因子的范围（最大EC与最小EC ₅₀之比））为46.03，表明所有野生型菌株均对SYP-14288敏感。为了检查抗真菌剂的风险，在用SYP-14288修改的琼脂平板上产生了20个抗SYP-14288的突变体。18个突变体在10次转移后仍保持抗性，其适应性与亲本菌株显着不同。所有的突变体生长都较慢，但对水稻植物显示出高毒力，尽管低于亲本菌株。交叉耐药性分析表明SYP-14288与具有或不具有与SYP-14288相同作用方式的杀真菌剂之间存在正相关关系，包括fluazinam，fentin chloride，fludioxonil，difenoconazole，cyazofamid，chlorothalonil和2,4 -二硝基苯。该结果表明由SYP-14288诱导的多药耐药性，茄红枯菌为常用的杀菌剂。