ABSTRACT: Using cultures of the SKF-9 cell line, megalocytivirus AFIV-16 was isolated from imported angelfish Pterophyllum scalare held in quarantine at the Australian border. The cytopathic effect caused by isolate AFIV-16 presented as cell rounding and enlargement, but complete destruction of the infected cell cultures did not occur. The infected cells demonstrated immunocytochemical reactivity with monoclonal antibody M10, which is used for diagnosis of OIE-listed red sea bream iridoviral disease. Using electron microscopy, the virus particles, consisting of hexagonal nucleocapsids, were observed in the cytoplasm of SKF-9 cells. The replication of AFIV-16 in cultured SKF-9 cells was significantly greater at 28°C incubation than at 22 and 25°C incubation, whereas no difference in growth characteristics was observed for red sea bream iridovirus (RSIV) isolate KagYT-96 across this temperature range. Whole genome sequencing demonstrated that AFIV-16 has a 99.96% similarity to infectious spleen and kidney necrosis virus (ISKNV), the type species in the genus Megalocytivirus. AFIV-16 was classified into ISKNV genotype Clade 1 by phylogenetic analysis of the major capsid protein gene nucleotide sequence. This is the first report of whole genome sequencing of an ISKNV genotype megalocytivirus isolated from ornamental fish.

中文翻译：

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从进口神仙鱼鳞翅目中分离出ISKNV基因型巨轮病毒的特征和特征。

摘要：使用SKF-9细胞系的培养物，megalocytivirus AFIV-16从进口神仙分离神仙鱼在澳大利亚边境隔离。由分离的AFIV-16引起的细胞病变作用表现为细胞四舍五入和增大，但是没有发生被感染细胞培养物的完全破坏。被感染的细胞表现出与单克隆抗体M10的免疫细胞化学反应，该单克隆抗体M10用于诊断OIE列出的红鲷虹吸病毒病。使用电子显微镜，在SKF-9细胞的细胞质中观察到由六边形核衣壳组成的病毒颗粒。在培养的SKF-9细胞中，AFIV-16的复制在28°C的条件下比在22和25°C的条件下的复制量要大得多，而红鲷鱼虹膜病毒（RSIV）分离的KagYT-96的生长特性没有观察到差异。这个温度范围。全基因组测序表明AFIV-16具有99。巨轮病毒。通过对主要衣壳蛋白基因核苷酸序列的系统进化分析，将AFIV-16分为ISKNV基因型1。这是从观赏鱼中分离出的ISKNV基因型巨轮病毒全基因组测序的首次报道。