In a conserved culture of the purple sulfur bacterium Thiospirillum jenense DSM216 T , cells of this species were easily recognized by cell morphology, large-size spirilla and visible flagellar tuft. The Tsp. jenense genome is 3.22 Mb in size and has a GC content of 48.7 mol%. It was readily identified as a member of the Chromatiaceae by the complement of proteins in its genome. A whole genome comparison clearly placed Tsp. jenense near Thiorhodovibrio and Rhabdochromatium species and somewhat more distant from Thiohalocapsa and Halochromatium species. This relationship was also found with the sequences of the photosynthetic reaction center protein PufM. The genome sequence supported important properties of this bacterium: the presence of ribulose-bisphosphate carboxylase and enzymes of the Calvin cycle of autotrophic carbon dioxide fixation but the absence of carboxysomes, an incomplete tricarboxylic acid cycle and the lack of malate dehydrogenase, the presence of a sulfur oxidation pathway including adenylylsulfate reductase ( aprAB ) but absence of assimilatory sulfate reduction, the presence of hydrogenase ( hoxHMFYUFE ), nitrogenase and a photosynthetic gene cluster ( pufBALMC ). The FixNOP type of cytochrome oxidase was notably lacking, which may be the reason that renders the cells highly sensitive to oxygen. Two minor phototrophic contaminants were found using metagenomic binning: one was identified as a strain of Rhodopseudomonas palustris and the second one has an average nucleotide identity of 82% to the nearest neighbor Rhodoferax antarcticus. It should be considered as a new species of this genus and Rhodoferax jenense is proposed as the name.

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巨型光养γ变形杆菌的基因组序列让我们深入了解其生理特性和系统发育关系

在紫色硫细菌 Thiospirillum jenense DSM216 T 的保守培养物中，该物种的细胞很容易通过细胞形态、大尺寸的螺旋体和可见的鞭毛簇识别。茶匙。jenense 基因组大小为 3.22 Mb，GC 含量为 48.7 mol%。通过其基因组中的蛋白质互补，它很容易被确定为色科的成员。全基因组比较清楚地放置了 Tsp。jenense 靠近 Thiorhodovibrio 和 Rhabdochromatium 物种，而与 Thiohalocapsa 和 Halochromatium 物种稍微远一些。这种关系也与光合反应中心蛋白 PufM 的序列有关。基因组序列支持这种细菌的重要特性：存在核酮糖-二磷酸羧化酶和自养二氧化碳固定的卡尔文循环酶，但不存在羧基体，不完整的三羧酸循环和缺乏苹果酸脱氢酶，存在硫氧化途径，包括腺苷酸还原酶 (aprAB) 但没有同化硫酸盐还原，存在氢化酶 (hoxHMFYUFE)、固氮酶和光合基因簇 (pufBALMC)。FixNOP 类型的细胞色素氧化酶明显缺乏，这可能是导致细胞对氧气高度敏感的原因。使用宏基因组分箱发现了两种轻微的光合污染物：一种被鉴定为沼泽红假单胞菌的菌株，另一种与最近的邻居南极红假单胞菌的平均核苷酸同一性为 82%。