In the present study, water-soluble hybrid selenium-containing nanocomposites have been synthesised via soft oxidation of selenide-anions, preliminarily generated from elemental bulk-selenium in the base-reduction system ‘N ₂ H ₄ –NaOH’. The nanocomposites obtained consist of Se ⁰ NPs (4.6–24.5 nm) stabilised by κ-carrageenan biocompatible polysaccharide. The structure of these composite nanomaterials has been proven using complementary physical–chemical methods: X-ray diffraction analysis, transmission electron microscopy, optical spectroscopy, and dynamic light scattering. Optical ranges of ‘emission/excitation’ of aqueous solutions of nanocomposites with Se ⁰ NPs of different sizes are established and the most important parameters of their luminescence are determined. For the obtained nanocomposites, the expressed antiradical activity against free radicals 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid has been found, the value of which depends on the size of selenium nanoparticles. It is experimentally revealed that all obtained nanocomposites are low toxic (LD ₅₀ >2000 mg/kg). It is also found that small selenium nanoparticles (6.8 nm), in contrast to larger nanoparticles (24.5 nm), are accumulated in organisms to significantly increase the level of selenium in the liver, kidneys, and brain (in lesser amounts) of rats.

中文翻译：

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不同大小的κ-角叉菜胶封端的硒纳米粒子的抗自由基活性、毒性和生物分布的合成和比较评估：体内和体外研究。

在本研究中，水溶性杂化含硒纳米复合材料是通过硒阴离子的软氧化合成的，硒阴离子是由碱还原系统“N ₂ H ₄ -NaOH”中的元素体硒初步生成的。 获得的纳米复合材料由由 κ-角叉菜胶生物相容性多糖稳定的 Se ^{0 NPs (4.6-24.5 nm) 组成。}这些复合纳米材料的结构已通过互补的物理化学方法得到证实：X 射线衍射分析、透射电子显微镜、光谱学和动态光散射。^{Se 0}纳米复合材料水溶液的“发射/激发”光学范围 建立了不同尺寸的纳米粒子，并确定了它们发光的最重要参数。对于获得的纳米复合材料，已发现对自由基 2,2-二苯基-1-苦基肼和 2,2'-叠氮基-双（3-乙基苯并噻唑啉-6-磺酸）的抗自由基活性，其值取决于硒纳米粒子的大小。实验表明，所有获得的纳米复合材料都是低毒性的 (LD ₅₀ >2000 mg/kg)。还发现，与较大的纳米粒子 (24.5 nm) 相比，小的硒纳米粒子 (6.8 nm)在生物体中积累，显着增加大鼠肝脏、肾脏和大脑（较少量）中的硒水平。