In this examination, we researched the advantages of DNA fragmentation and metallic nanoparticles well-appointed with biomolecules. A novel interpretation of DNA damage by Silver Nano-Clusters (AgNCs) which were developed by the utilization of green synthesis method was demonstrated. The green synthesis of AgNCs was accomplished by utilizing the leaf extract of Salacia mulbarica (SM). The preparation of SM-AgNCs was developed by estimating surface plasmon resonance peak around 449 nm by using a UV–Visible spectrophotometer. The effect of phytochemicals in SM leaf extract on the development of stable SM-AgNCs was confirmed by FTIR spectroscopy. The size of the fabricated SM-AgNCs was estimated by dynamic light scattering and zeta-sizer analysis and the morphology of the SM-AgNCs was examined by transmission electron microscopy. The presence of clusters of Ag particles in the prepared SM-AgNCs was recognized by energy dispersion X-ray analysis. The results show that saponins, phytosterols, and phenolic compounds present in plant extract may play a great part in developing the SM-AgNCs in their specialized particles. The succeeded SM-AgNCs shows incredible anti-bacterial action towards Escherichia coli and Bacillus subtilis . In-light of the antibacterial study, these SM-AgNCs were analyzed with calf thymus-DNA and found significant damage to the strand of thymus-DNA.

中文翻译：

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五层龙叶提取物通过释放活性氧来介导银纳米粒子的合成，用于抗菌和 ct-DNA 损伤。

在本次考试中，我们研究了 DNA 片段化和金属纳米粒子与生物分子相匹配的优势。展示了利用绿色合成方法开发的银纳米簇 (AgNCs) 对 DNA 损伤的新解释。AgNCs的绿色合成是利用叶提取物完成的。五层龙（SM）。通过使用紫外-可见分光光度计估计 449 nm 附近的表面等离子体共振峰来开发 SM-AgNCs 的制备。FTIR 光谱证实了 SM 叶提取物中植物化学物质对稳定 SM-AgNCs 发育的影响。通过动态光散射和 zeta-sizer 分析估计制造的 SM-AgNCs 的大小，并通过透射电子显微镜检查 SM-AgNCs 的形态。通过能量色散 X 射线分析，在制备的 SM-AgNCs 中存在 Ag 粒子簇。结果表明，植物提取物中存在的皂苷、植物甾醇和酚类化合物可能在其特殊颗粒中开发 SM-AgNCs 中发挥重要作用。成功的 SM-AgNCs 显示出令人难以置信的抗菌作用大肠杆菌和枯草芽孢杆菌 . 根据抗菌研究，这些 SM-AgNCs 用小牛胸腺-DNA 进行分析，发现胸腺-DNA 链有显着损伤。