Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Successfully isolated and cultured DPCs of Rex rabbit could provide a good model for the study of hair follicle development mechanism in vitro. Skin samples were collected from 30-day old Rex rabbits and separated by combination of Dispase II and Collagenase D, separation, culture, and purification of DPCs. The morphology of DPCs in vitro was observed and the growth curve was drawn, the number of DPCs presented progressive increase in a logarithmic model between the 4th day and the 7th day. The results of immune chemical and immune fluorescence shown that α smooth muscle actin (α-SMA) and versican were positive in cells. Growth character of the passages 3 (P3), P6, P9 and P12 DPCs were observed using MTT at 24 h, 48 h, 72 h, 96 h, 120 h and 144 h. The cell density of P12 was lower than P3 (P < 0.05); the flow cytometric analysis showed that DPCs at resting state/first gap (G0/G1) stage of P3 was higher than P12 (P < 0.05), and second gap/mitosis (G2/M) stage of P3 was lower than P12 (P < 0.05). However, the DPCs of P12 present triangular or short fusiform, retaining their unique aggregative growth characteristics. This results shown that the DPCs properties of P12 from Rex rabbits, still fit functional research in vitro. In conclusion, we successfully established the culturing condition of DPCs from Rex rabbits, and provide a material for studying the molecular mechanism of hair follicle development.

中文翻译：

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雷克斯兔真皮乳头细胞的分离，培养和生长特性。

真皮乳头细胞（DPC）是毛囊的主要皮肤成分，直接调节毛囊的发育，生长和再生。成功地分离和培养了獭兔DPCs，可以为体外研究毛囊发育机理提供良好的模型。从30天大的獭兔中收集皮肤样品，并通过Dispase II和胶原酶D的组合，DPC的分离，培养和纯化进行分离。观察了DPC的体外形态并绘制了生长曲线，在对数模型中在第4天至第7天之间，DPC的数量呈逐渐增加的趋势。免疫化学和免疫荧光的结果表明，细胞中的α平滑肌肌动蛋白（α-SMA）和versican呈阳性。段落3（P3），P6，使用MTT在24小时，48小时，72小时，96小时，120小时和144小时观察到P9和P12 DPC。P12的细胞密度低于P3（P <0.05）；流式细胞仪分析显示，P3在静息状态/第一间隙（G0 / G1）阶段的DPC高于P12（P <0.05），P3的第二间隙/有丝分裂（G2 / M）阶段低于P12（P <0.05）。但是，P12的DPC呈三角形或短梭形，保留了其独特的聚集生长特性。该结果表明，来自獭兔的P12的DPCs特性仍然适合体外功能研究。综上所述，我们成功建立了獭兔DPCs的培养条件，为研究毛囊发育的分子机理提供了材料。0.05）；流式细胞仪分析显示，P3在静息状态/第一间隙（G0 / G1）阶段的DPC高于P12（P <0.05），P3的第二间隙/有丝分裂（G2 / M）阶段低于P12（P <0.05）。但是，P12的DPC呈三角形或短梭形，保留了其独特的聚集生长特性。该结果表明，来自獭兔的P12的DPCs特性仍然适合体外功能研究。综上所述，我们成功地建立了獭兔DPCs的培养条件，为研究毛囊发育的分子机理提供了材料。0.05）；流式细胞仪分析显示，P3在静息状态/第一间隙（G0 / G1）阶段的DPC高于P12（P <0.05），P3的第二间隙/有丝分裂（G2 / M）阶段低于P12（P <0.05）。但是，P12的DPC呈三角形或短梭形，保留了其独特的聚集生长特性。该结果表明，来自獭兔的P12的DPCs特性仍然适合体外功能研究。综上所述，我们成功建立了獭兔DPCs的培养条件，为研究毛囊发育的分子机理提供了材料。P12的DPC呈三角形或短梭形，保留了其独特的聚集生长特征。该结果表明，来自獭兔的P12的DPCs特性仍然适合体外功能研究。综上所述，我们成功建立了獭兔DPCs的培养条件，为研究毛囊发育的分子机理提供了材料。P12的DPC呈三角形或短梭形，保留了其独特的聚集生长特征。该结果表明，来自獭兔的P12的DPCs特性仍然适合体外功能研究。综上所述，我们成功建立了獭兔DPCs的培养条件，为研究毛囊发育的分子机理提供了材料。