The suppressive roles of limonin have been established in various tumors. However, its roles in oral tongue squamous cell carcinoma (OTSCC) progression are still confusing. This work aims to explore limonin-mediated effects on OTSCC progression. CCK8 analysis was performed to evaluate limonin-mediated effects on OTSCC cell viability. Wound healing and transwell invasion analysis were constructed to examine the effects of limonin on OTSCC cell migration and invasion capacity. RT-qPCR, western blot and luciferase reporter assays were used to explore the underlying mechanisms contributing to limonin-mediated effects on OTSCC progression. It was found that limonin significantly suppressed the viability of OTSCC cells. Additionally, limonin reduced the migration and invasion ability of OTSCC cells. Mechanistically, limonin suppresses OTSCC progression by promoting the nuclear to cytoplasm translocation of YAP, decreasing YAP protein expression and subsequently decreasing YAP transcriptional regulatory activity, this is responsible for limonin-mediated suppression on OTSCC progression. Thus, this work suggests that limonin suppresses OTSCC progression through inhibiting YAP transcriptional regulatory activity.

中文翻译：

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柠檬苦素通过抑制YAP转录调控活性来抑制口腔鳞状细胞癌的发展。

柠檬苦素的抑制作用已在多种肿瘤中确立。但是，它在口腔鳞状细胞癌（OTSCC）进展中的作用仍然令人困惑。这项工作旨在探讨柠檬苦素介导的对OTSCC进展的影响。进行CCK8分析以评估柠檬苦素介导的对OTSCC细胞活力的影响。构建伤口愈合和transwell侵袭分析，以研究柠檬苦素对OTSCC细胞迁移和侵袭能力的影响。使用RT-qPCR，Western印迹和荧光素酶报告基因检测来探讨柠檬苦素介导的对OTSCC进程影响的潜在机制。发现柠檬苦素显着抑制了OTSCC细胞的活力。另外，柠檬苦素降低了OTSCC细胞的迁移和侵袭能力。机械上，柠檬苦素通过促进YAP的核向细胞质易位，降低YAP蛋白表达并随后降低YAP转录调节活性来抑制OTSCC进程，这是柠檬苦素介导的对OTSCC进程的抑制。因此，这项工作表明柠檬苦素通过抑制YAP转录调节活性来抑制OTSCC进展。