Currently, the confirmation of diagnosis of breast cancer is made by microscopic examination of an ultra-thin slice of a needle biopsy specimen. This slice is conventionally formalin-fixed and stained with hematoxylin-eosin and visually examined under a light microscope. This process is labor-intensive and requires highly skilled doctors (pathologists). In this paper, we report a novel tool based on near-infrared spectroscopy (Spectral-IRDx) which is a portable, non-contact, and cost-effective system and could provide a rapid and accurate diagnosis of cancer. The Spectral-IRDx tool performs absorption spectroscopy at near-infrared (NIR) wavelengths of 850, 935, and 1060 nm. We measure normalized detected voltage (V _dn ) with the tool in 10 deparaffinized breast biopsy tissue samples, 5 of which were cancer (C) and 5 were normal (N) tissues. The difference in V _dn at 935 nm and 1060 nm between cancer and normal tissues is statistically significant with p -values of 0.0038 and 0.0022 respectively. Absorption contrast factor (N/C) of 1.303, 1.551, and 1.45 are observed for 850, 935, and 1060 nm respectively. The volume fraction contrast (N/C) of lipids and collagens are reported as 1.28 and 1.10 respectively. Higher absorption contrast factor (N/C) and volume fraction contrast (N/C) signifies higher concentration of lipids in normal tissues as compared to cancerous tissues, a basis for delineation. These preliminary results support the envisioned concept for noninvasive and noncarcinogenic NIR-based breast cancer diagnostic platform, which will be tested using a larger number of samples.

中文翻译：

---

建立与多光谱非接触式探头集成的便携式平台，用于基于近红外光谱描绘正常组织和乳腺癌组织


目前，乳腺癌的诊断是通过对针吸活检标本的超薄切片进行显微镜检查来确认的。该切片通常用福尔马林固定并用苏木精-伊红染色，并在光学显微镜下进行目视检查。这个过程是劳动密集型的，需要高技能的医生（病理学家）。在本文中，我们报告了一种基于近红外光谱（Spectral-IRDx）的新型工具，它是一种便携式、非接触式且具有成本效益的系统，可以提供快速、准确的癌症诊断。 Spectral-IRDx 工具可在 850、935 和 1060 nm 的近红外 (NIR) 波长下执行吸收光谱分析。我们使用该工具在 10 个脱蜡的乳腺活检组织样本中测量标准化检测电压 (V _dn )，其中 5 个是癌症 (C) 组织，5 个是正常 (N) 组织。癌症和正常组织之间在 935 nm 和 1060 nm 处的 V _dn差异具有统计学显着性，p 值分别为 0.0038 和 0.0022。在 850 nm、935 nm 和 1060 nm 处观察到的吸收对比度 (N/C) 分别为 1.303、1.551 和 1.45。脂质和胶原蛋白的体积分数对比 (N/C) 分别报告为 1.28 和 1.10。较高的吸收对比因子 (N/C) 和体积分数对比 (N/C) 表示正常组织中的脂质浓度高于癌组织，这是描绘的基础。这些初步结果支持基于近红外的非侵入性和非致癌性乳腺癌诊断平台的设想概念，该平台将使用大量样本进行测试。