Endophytes are considered one of the most important microbial resources for obtaining biomolecules of therapeutic use. Passiflora incarnata, widely employed by the pharmaceutical industry, shows therapeutic effects on anxiety, nervousness, constipation, dyspepsia and insomnia based on their antioxidant compounds. In this study, from 315 endophytic fungi isolated from P. incarnata leaves, 60 were selected to determinate presence of chemical constituents related with antioxidant activity, based on their production of soluble pigments. The promising fungi were evaluated specifically on their potential to produce phenolic compounds, flavonoids and for antioxidant activity. Five isolates significantly produced flavonoids and phenolic compounds in the ethyl acetate and n-Butanol extracts, also saponins and high antioxidant activity against the DPPH (2.2-diphenyl-1-picrylhydrazyl) free radical. A strain of Aspergillus nidulans var. dentatus (former Emericella dentata) was able to produce tannins as well; its butanolic extract was very similar than the BHT (butylated hydroxytoluene) (94.3% × 94.32%) and Rutin (95.8%) reference substances in the DPPH radical scavenging. Similarly, a Chaetomium strain exhibited 93.6% and 94.7% of antioxidant activity in their ethyl acetate and butanolic fractions, respectively. The chromatographic analysis of the ethyl acetate fraction from the Aspergillus strain revealed the production of orcinol (3.19%). Four-methoxymethylphenol (4.79%), sorbicillin (33.59%) and ergosterol (23.08%) was produced by Trichoderma longibrachiatum and isopropenyl-1,4-dimethyl-1,2,3,3a,4,5,6,7-octahydroazulene were found in two Fusarium oxysporum strains. The phytochemical screening showed that all analyzed fungi were able to produce a kind of secondary metabolite (phenols, flavonoids, tannins and/or saponins). The study shows a great underexplored potential for industrial application of P. incarnata endophytes.

中文翻译：

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来自 Passiflora incarnata 的内生真菌：一种抗氧化化合物来源

内生菌被认为是获得治疗用途的生物分子的最重要的微生物资源之一。Passiflora incarnata 被制药行业广泛采用，其抗氧化化合物对焦虑、紧张、便秘、消化不良和失眠具有治疗作用。在这项研究中，从 P. incarnata 叶子中分离出的 315 种内生真菌中，选择了 60 种，根据它们产生的可溶性色素来确定与抗氧化活性相关的化学成分的存在。这些有前途的真菌被专门评估了它们产生酚类化合物、类黄酮和抗氧化活性的潜力。五个分离物在乙酸乙酯和正丁醇提取物中显着产生类黄酮和酚类化合物，还有皂苷和对 DPPH 的高抗氧化活性 (2. 2-diphenyl-1-picrylhydrazyl) 自由基。构巢曲霉变种的菌株。dentatus（原Emericella dentata）也能产生单宁；其丁醇提取物与 BHT（丁基化羟基甲苯）（94.3% × 94.32%）和芦丁（95.8%）参比物质在 DPPH 自由基清除方面非常相似。类似地，毛壳菌菌株在其乙酸乙酯和丁醇级分中分别表现出 93.6% 和 94.7% 的抗氧化活性。来自曲霉属菌株的乙酸乙酯级分的色谱分析表明产生了地黄酚 (3.19%)。四甲氧基甲基苯酚 (4.79%)、山梨西林 (33.59%) 和麦角甾醇 (23.08%) 由长柄木霉和异丙烯基-1,4-二甲基-1,2,3,3a,4,5,6,7-octahydroazulene 产生在两种尖孢镰刀菌菌株中发现。植物化学筛选表明，所有分析的真菌都能够产生一种次级代谢物（酚类、黄酮类、单宁和/或皂苷）。该研究显示了 P. incarnata 内生菌在工业应用方面的巨大未开发潜力。