This 24‐color flow cytometry panel focuses on characterizing antigen‐specific B cells and precise delineation of B‐cell subsets in chronic infections and is applicable to other chronic diseases such as autoimmunity. The panel was optimized for human cryopreserved peripheral blood mononuclear cells (PBMCs). Markers were chosen to extensively distinguish B‐cell lineages (CD19, CD20, CD10, CD38, CD24, IgM, IgD, CD27, CD21, CD43, CD5). Inclusion of antigen‐specific probes was of high priority in order to assess hepatitis B virus (HBV) antigen‐specific B cells for our purposes. These probes can be readily exchanged for other pathogen‐specific probes or additional markers for the panel to be tailored to desired research questions beyond HBV. In addition, we included a comprehensive and unique set of functional markers such as chemokine receptors (CXCR3, CXCR5), co‐stimulatory molecule (CD86), Fc receptor (CD32), regulatory molecules (BTLA, CD39), and inhibitory markers associated with chronic infections (PD‐1, FcRL5, CD11c, CD22) to enable in‐depth analysis of global and antigen‐specific B cells during chronic infection. © 2020 International Society for Advancement of Cytometry

中文翻译：

---

OMIP-068：慢性感染中全局和抗原特异性 B 细胞的高维表征。

这个 24 色流式细胞术面板专注于表征抗原特异性 B 细胞和慢性感染中 B 细胞亚群的精确描绘，适用于其他慢性疾病，如自身免疫。该面板针对人类冷冻保存的外周血单核细胞 (PBMC) 进行了优化。选择标记来广泛区分 B 细胞谱系（CD19、CD20、CD10、CD38、CD24、IgM、IgD、CD27、CD21、CD43、CD5）。为了我们的目的评估乙型肝炎病毒 (HBV) 抗原特异性 B 细胞，包含抗原特异性探针是高度优先事项。这些探针可以很容易地与其他病原体特异性探针或其他标记物交换，以针对 HBV 之外的所需研究问题进行定制。此外，我们还包括了一套全面而独特的功能标记，例如趋化因子受体 (CXCR3、CXCR5)、共刺激分子 (CD86)、Fc 受体 (CD32)、调节分子 (BTLA、CD39) 和与慢性感染相关的抑制标志物 (PD-1、FcRL5、CD11c、CD22) 以实现对慢性感染期间的全局和抗原特异性 B 细胞。© 2020 国际细胞计量学促进会