Acrylamide/chitosan-based cryogel was fabricated, and a triazine dye, Reactive Green 5, was attached to the cryogel by nucleophilic substitution to build a dye affinity support for adsorption of catalase enzyme. Characterization of cryogel was performed using FTIR, SEM, EDX, BET, and swelling test. Synthesized cryogel beared pores with ~ 200 μm in size and the surface area of 11.8 m²/g. Maximum catalase adsorption was (17.6 ± 0.29 mg/g) measured at pH 4.0 and 25 °C. The adsorption sites on the cryogel were saturated at 0.75 mg/mL enzyme concentration. Increased ionic strength caused a decrease in adsorption capacity. Desorption of catalase from cryogel was enabled using 0.5 M NaSCN solution. Consecutive adsorption experiments were carried out fifteen times to evaluate the reusability of the cryogel. Thermal, storage, and operational stabilities of immobilized catalase were higher than the free one. The data produced implicate that catalase-adsorbed dye-affinity cryogel may be used for H₂O₂ detection or removal when necessary.

制备了基于丙烯酰胺/壳聚糖的冷冻凝胶，并通过亲核取代将三嗪染料 Reactive Green 5 连接到冷冻凝胶上，以构建吸附过氧化氢酶的染料亲和支持。使用 FTIR、SEM、EDX、BET 和溶胀试验对冷冻凝胶进行表征。合成的带有约 200 μm 大小和表面积 11.8 m ^{2 的}冷冻凝胶孔/G。在 pH 4.0 和 25 °C 下测得的最大过氧化氢酶吸附为 (17.6 ± 0.29 mg/g)。冷冻凝胶上的吸附位点在 0.75 mg/mL 酶浓度下饱和。离子强度增加导致吸附能力下降。使用 0.5 M NaSCN 溶液可以从冷冻凝胶中解吸过氧化氢酶。连续进行十五次吸附实验以评估冷冻凝胶的可重复使用性。固定化过氧化氢酶的热稳定性、储存稳定性和操作稳定性均高于游离的。产生的数据表明，吸附过氧化氢酶的染料亲和冷冻凝胶可在必要时用于 H ₂ O ₂检测或去除。