The effects of bulk- and nano-CuO were monitored on barley (Hordeum sativum L.) in hydroponic conditions. The anatomical and cyto-/morphometric parameters of plants, exposed to both types of CuO in different doses (300 and 2000 mg/L) were recorded. The germination rate, root and shoot lengths decreased in a dose-dependent manner. Exposure to nano-CuO significantly increased Cu content in the H. sativum roots; however, the translocation rates of dissolved Cu were low and showed less accumulation in above-ground tissues. The differences between nano- and bulk-CuO treated plants were sufficiently evident, but at lower concentrations, these differences were non-significant. The relative seed germination inhibition was noted up to 11% and 22% under the high dose of bulk- and nano-CuO, respectively; however, at low dose, it was non-significant. The relative root length was reduced 3.6 fold by bulk- and 1.5 fold by nano-CuO, and shoot lengths decreased 1.6 fold by bulk- and 1.4 fold by nano-CuO under the high dose after growth of 30 days. It indicated more morphological effects on H. sativum caused by bulk- than the nano-CuO. The cytomorphometric analysis indicated the average cortex cell, total cortex, and total central cylinder areas of root cells and the average areas of chlorenchyma leaf cells were increased as compared to control in both bulk- and nano-CuO treated plants. It showed destructive effects of nano- and bulk-CuO on cellular organizations of H. sativum anatomy. Thus, at the low dose, the minimal effects of nano-CuO were observed than the bulk. Therefore, the finding could be interest for the safe application of nano-CuO.

在水培条件下对大麦 ( Hordeum sativum L.)监测块状和纳米 CuO 的影响。记录暴露于不同剂量（300 和 2000 毫克/升）的两种类型的 CuO 的植物的解剖学和细胞/形态学参数。发芽率、根和芽长度以剂量依赖性方式降低。暴露于纳米 CuO 显着增加了H. sativum中的 Cu 含量根；然而，溶解铜的易位率较低，在地上组织中积累较少。纳米和块状 CuO 处理的植物之间的差异非常明显，但在较低浓度下，这些差异不显着。在高剂量的块状和纳米 CuO 下，相对种子萌发抑制率分别高达 11% 和 22%；然而，在低剂量下，它是不显着的。生长 30 天后，在高剂量下，相对根长减少了 3.6 倍，纳米 CuO 减少了 1.5 倍，芽长减少了 1.6 倍，纳米 CuO 减少了 1.4 倍。它表明对H. sativum 有更多的形态学影响引起的块状比纳米CuO。细胞形态计量学分析表明，与对照相比，在块状和纳米 CuO 处理的植物中，根细胞的平均皮层细胞、总皮层和总中央圆柱面积以及绿藻叶细胞的平均面积均增加。它显示了纳米和块状 CuO 对H. sativum解剖结构的细胞组织的破坏作用。因此，在低剂量下，观察到纳米 CuO 的影响最小。因此，该发现可能对纳米 CuO 的安全应用感兴趣。