Flaviviruses are a group of important emerging and re-emerging human pathogens that cause worldwide epidemics with thousands of deaths annually. Flaviviruses are small, enveloped, positive-sense, single-stranded RNA viruses that are obligate intracellular pathogens, relying heavily on host cell machinery for productive replication. Proteomic approaches have become an increasingly powerful tool to investigate the mechanisms by which viruses interact with host proteins and manipulate cellular processes to promote infection. Here, we review recent advances in employing quantitative proteomics techniques to improve our understanding of the complex interplay between flaviviruses and host cells. We describe new findings on our understanding of how flaviviruses impact protein-protein interactions, protein-RNA interactions, protein abundance, and post-translational modifications to modulate viral infection.

中文翻译：

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使用定量蛋白质组学破译黄病毒-宿主相互作用。

黄病毒是一组重要的新出现和重新出现的人类病原体，每年会在全球范围内引起数千人死亡。黄病毒是小的、有包膜的、正义的、单链 RNA 病毒，它们是专性细胞内病原体，严重依赖宿主细胞机制进行生产性复制。蛋白质组学方法已成为研究病毒与宿主蛋白质相互作用和操纵细胞过程以促进感染的机制的越来越强大的工具。在这里，我们回顾了采用定量蛋白质组学技术来提高我们对黄病毒和宿​​主细胞之间复杂相互作用的理解的最新进展。我们描述了关于黄病毒如何影响蛋白质-蛋白质相互作用、蛋白质-RNA 相互作用、蛋白质丰度、