OBJECTIVE Recurrent pregnancy loss (RPL) has been associated with thrombophilia. The use of prophylactic treatments against thrombophilia becomes necessary in order to increase the live birth rates in women with RPL. The aim of this study was to genotype thrombophilia associated polymorphisms and investigates the benefit of prophylactic treatment on the clinical pregnancy outcomes of women with specific genotypes of these polymorphisms. MATERIALS AND METHODS A total of 62 women were included in this study. The polymorphisms associated with thrombophilia, including methyltetrahydrofolate reductase (MTHFR) 1298 and 677, Factor V Leiden (FVL) 1691, plasminogen activator inhibitor-1 (PA1-1) G/G and Factor II prothrombin 20,210, were genotyped using the real time PCR. The effect of prophylactic treatment using anti-coagulants of 0.4 mL dose of enoxaparin (3000-6000IU) and 75 mg dose of aspirin, 81 mg dose of aspirin, mineral of 15 mg dose of zinco c or10 mg dose of folic acid, was correlated with the genotypes of polymorphisms. RESULTS AND CONCLUSION The clinical pregnancy outcomes were significantly improved in patients with MTHFR 677CC genotype when treated with zinco c. Furthermore, treatment with 75 mg of aspirin resulted in higher negative pregnancy rates in patients with MTHFR A1298C genotypes. Therefore, the results of this study should be used to re-evaluate the clinical applications in women with miscarriages.

中文翻译：

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血栓形成相关基因多态性：使用药物，包括抗凝血剂、矿物质或叶酸，是否可以预防流产？

目的 复发性流产 (RPL) 与血栓形成倾向有关。为了提高 RPL 妇女的活产率，有必要对血栓形成倾向进行预防性治疗。本研究的目的是对血栓形成倾向相关的多态性进行基因分型，并研究预防性治疗对具有这些多态性特定基因型的女性的临床妊娠结局的益处。材料和方法 本研究共包括 62 名女性。与血栓形成倾向相关的多态性，包括甲基四氢叶酸还原酶 (MTHFR) 1298 和 677、因子 V Leiden (FVL) 1691、纤溶酶原激活剂抑制剂-1 (PA1-1) G/G 和因子 II 凝血酶原 20,210，使用实时 PCR 进行基因分型. 使用抗凝剂进行预防性治疗的效果为 0。4 mL剂量的依诺肝素（3000-6000IU）和75 mg剂量的阿司匹林、81 mg剂量的阿司匹林、15 mg剂量的锌钙或10 mg剂量的叶酸与多态性的基因型相关。结果与结论 MTHFR 677CC 基因型患者接受锌治疗后，临床妊娠结局显着改善。此外，使用 75 mg 阿司匹林治疗导致 MTHFR A1298C 基因型患者的阴性妊娠率更高。因此，本研究的结果应用于重新评估流产妇女的临床应用。结果与结论 MTHFR 677CC 基因型患者接受锌治疗后，临床妊娠结局显着改善。此外，使用 75 mg 阿司匹林治疗导致 MTHFR A1298C 基因型患者的阴性妊娠率更高。因此，本研究的结果应用于重新评估流产妇女的临床应用。结果与结论 MTHFR 677CC 基因型患者接受锌治疗后，临床妊娠结局显着改善。此外，使用 75 mg 阿司匹林治疗导致 MTHFR A1298C 基因型患者的阴性妊娠率更高。因此，本研究的结果应用于重新评估流产妇女的临床应用。