Stroke is partial or complete brain dysfunction combined with acute cerebral circulatory disorders, and it affects millions of individuals around the world each year. A total of 70-80% of patients experience ischaemic stroke caused by disturbances in cerebral circulation, leading to cerebral ischaemia, neuronal apoptosis, and necrosis. Tanshinone IIA is a natural compound extracted from Salvia miltiorrhiza and has been proven to assist in recovery from cerebral ischaemia reperfusion injury. GLUT1 is ubiquitously expressed in all types of tissues in the human body and has important physiological functions due to its glucose uptake ability. This experiment was performed to detect the effect of GLUT1 in promoting the therapeutic effect of tanshinone IIA. Here, we found that tanshinone IIA treatment increased the viability of neurons and promoted the recovery of brain function, and that the concentration of glucose in serum and cultured medium was also increased. We noticed that these effects might be mediated by an increased glucose uptake ability. In addition, we further found that the PI3K/mTOR/HER3 signalling pathway played an important role in regulating these effects. Thus, we thought that overexpression of GLUT1 might be an important target in the treatment of cerebral ischaemia-reperfusion.

中文翻译：

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丹参酮IIA通过在缺血再灌注模型（体内和体外实验）中靶向GLUT1减轻神经细胞的损伤。

中风是部分或完全的脑功能障碍，并伴有急性的脑循环障碍，每年影响全世界数以百万计的人。共有70-80％的患者经历由脑循环障碍引起的缺血性中风，从而导致脑缺血，神经元凋亡和坏死。丹参酮IIA是从丹参中提取的天然化合物，并已被证明可以帮助从脑缺血再灌注损伤中恢复。GLUT1在人体的所有类型的组织中普遍表达，并且由于其葡萄糖摄取能力而具有重要的生理功能。进行该实验以检测GLUT1在促进丹参酮IIA的治疗作用中的作用。这里，我们发现，丹参酮IIA处理可增加神经元的活力并促进脑功能的恢复，并且血清和培养基中葡萄糖的浓度也会增加。我们注意到，这些影响可能是由增加的葡萄糖摄取能力介导的。此外，我们进一步发现PI3K / mTOR / HER3信号通路在调节这些作用中起着重要作用。因此，我们认为GLUT1的过表达可能是治疗脑缺血再灌注的重要靶标。我们进一步发现，PI3K / mTOR / HER3信号通路在调节这些作用中起着重要作用。因此，我们认为GLUT1的过表达可能是治疗脑缺血再灌注的重要靶标。我们进一步发现PI3K / mTOR / HER3信号通路在调节这些作用中起着重要作用。因此，我们认为GLUT1的过表达可能是治疗脑缺血再灌注的重要靶标。