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Loss of Heterozygosity and Base Mutation Rates Vary Among Saccharomyces cerevisiae Hybrid Strains.
G3: Genes, Genomes, Genetics ( IF 2.1 ) Pub Date : 2020-09-01 , DOI: 10.1534/g3.120.401551
Ajith V Pankajam 1 , Suman Dash 1 , Asma Saifudeen 1 , Abhishek Dutta 1 , Koodali T Nishant 2
Affiliation  

A growing body of evidence suggests that mutation rates exhibit intra-species specific variation. We estimated genome-wide loss of heterozygosity (LOH), gross chromosomal changes, and single nucleotide mutation rates to determine intra-species specific differences in hybrid and homozygous strains of Saccharomyces cerevisiae. The mutation accumulation lines of the S. cerevisiae hybrid backgrounds - S288c/YJM789 (S/Y) and S288c/RM11-1a (S/R) were analyzed along with the homozygous diploids RM11, S288c, and YJM145. LOH was extensive in both S/Y and S/R hybrid backgrounds. The S/Y background also showed longer LOH tracts, gross chromosomal changes, and aneuploidy. Short copy number aberrations were observed in the S/R background. LOH data from the S/Y and S/R hybrids were used to construct a LOH map for S288c to identify hotspots. Further, we observe up to a sixfold difference in single nucleotide mutation rates among the S. cerevisiae S/Y and S/R genetic backgrounds. Our results demonstrate LOH is common during mitotic divisions in S. cerevisiae hybrids and also highlight genome-wide differences in LOH patterns and rates of single nucleotide mutations between commonly used S. cerevisiae hybrid genetic backgrounds.



中文翻译:

酿酒酵母杂种菌株之间杂合性的丧失和碱基突变率的变化。

越来越多的证据表明,突变率表现出种内特异性变异。我们估计杂合度(LOH),总染色体变化和单核苷酸突变率的全基因组损失,以确定酿酒酵母的杂种和纯合菌株的种内特异性差异。酿酒酵母的突变积累系杂种背景-分析了S288c / YJM789(S / Y)和S288c / RM11-1a(S / R)以及纯合二倍体RM11,S288c和YJM145。LOH在S / Y和S / R混合背景中都有广泛的应用。S / Y背景还显示出更长的LOH区域,总体染色体变化和非整倍性。在S / R背景中观察到短拷贝数畸变。来自S / Y和S / R杂种的LOH数据用于构建S288c的LOH图,以识别热点。此外,我们观察到酿酒酵母S / Y和S / R遗传背景之间的单核苷酸突变率差异高达六倍。我们的结果表明,LOH在酿酒酵母的有丝分裂分裂中很常见杂种,也突出了常用酿酒酵母杂种遗传背景之间LOH模式和单核苷酸突变率的全基因组差异。

更新日期:2020-09-02
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