Objective. Gel aspiration-ejection (GAE) has recently been developed for the rapid production of dense, anisotropic collagen gel scaffolds with adjustable collagen fibrillar densities. In this study, a GAE system was applied to produce aligned Schwann cells within a type-1 collagen matrix to generate GAE-engineered neural tissues (GAE-EngNT) for potential nerve tissue engineering applications. Approach. The stability and mechanical properties of the constructs were investigated along with the viability, morphology and distribution of Schwann cells. Having established the methodology to construct stable robust Schwann cell-loaded engineered neural tissues using GAE (GAE-EngNTs), the potential of these constructs in supporting and guiding neuronal regeneration, was assessed both in vitro and in vivo. Main results. Dynamic mechanical analysis strain and frequency sweeps revealed that the GAE-EngNT produced via cannula gauge number 16 G (∼1.2 mm diameter...

中文翻译：

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快速形成的稳定且对齐的致密胶原蛋白凝胶接种有施万细胞，可支持周围神经再生。

目的。凝胶抽吸-喷射（GAE）最近已被开发出来，用于快速生产具有可调节胶原纤维密度的致密各向异性胶原凝胶支架。在这项研究中，将GAE系统应用于在1型胶原蛋白基质中产生对齐的Schwann细胞，以生成GAE改造的神经组织（GAE-EngNT），以用于潜在的神经组织工程应用。方法。研究了构建体的稳定性和机械性能以及雪旺细胞的活力，形态和分布。建立了使用GAE（GAE-EngNTs）构建稳定的，稳定的，施旺旺细胞加载的工程神经组织的方法学后，在体外和体内评估了这些构建体在支持和指导神经元再生中的潜力。主要结果。