Introduction. Globally, human bocavirus (HBoV) has been detected in respiratory samples from patients suffering from upper and lower respiratory diseases. In Kuwait, little is known about the epidemiological and clinical characterization of the virus and genetic characterization of the virus as a respiratory pathogen is unknown. Aim. This study aims to explore the molecular epidemiology and clinical features of HBoV isolates in patients with respiratory diseases. Methodology. Retrospectively, between 2018 and 2020, 5941 respiratory samples from patients with respiratory diseases were screened for respiratory viruses using multiplex real-time PCR. Samples that were positive for HBoV were then subjected to NP1 and VP1/PV2 phylogenetic analysis. Results. HBoV was detected in 1.9 % of the patients, with a peak incidence of infection among children <1 year old. Co-infection with other respiratory viruses was observed in 56.8 % of HBoV-positive patients. Fever, cough and respiratory distress were the most common clinical features of HBoV infection. Phylogenetic analysis of the Kuwaiti HBoV isolates revealed that all the isolates were of the HBoV-1 genotype, with slight sequence variations among the isolates. Conclusion. This study illustrated the predominance of the HBoV-1 genotype in patients with respiratory diseases in Kuwait with minimal genetic variability. It also highlighted the clinical features of HBoV-1 infection, verifying its role in respiratory diseases.

中文翻译：

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科威特的人类博卡病毒（HBoV）：呼吸系统疾病患者中该病毒的分子流行病学和临床结果。


介绍。在全球范围内，已在患有上呼吸道和下呼吸道疾病的患者的呼吸道样本中检测到人类博卡病毒（HBoV）。在科威特，人们对该病毒的流行病学和临床特征知之甚少，并且该病毒作为呼吸道病原体的遗传特征尚不清楚。目的。本研究旨在探讨呼吸系统疾病患者中HBoV分离株的分子流行病学和临床特征。方法论。回顾2018年至2020年，利用多重实时PCR对5941份呼吸道疾病患者的呼吸道样本进行了呼吸道病毒筛查。然后对 HBoV 呈阳性的样本进行 NP1 和 VP1/PV2 系统发育分析。结果。 1.9%的患者检出HBoV，其中儿童感染率最高<1 id=4>结论。这项研究说明了科威特呼吸系统疾病患者中 HBoV-1 基因型的优势，遗传变异性极小。它还强调了HBoV-1感染的临床特征，验证了其在呼吸道疾病中的作用。