Bioprocess and Biosystems Engineering ( IF 3.5 ) Pub Date : 2020-07-28 , DOI: 10.1007/s00449-020-02412-7 Helena González-Peñas 1 , Thelmo A Lu-Chau 1 , Gemma Eibes 1 , Juan M Lema 1
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Abstract
The reindustrialization of acetone–butanol–ethanol (ABE) fermentation is hampered by its significant production cost, linked to high product inhibition and low product yield. ABE fermentation can be significantly enhanced by integrating in situ liquid–liquid extraction. In this study, hybrid simulations using Excel® and ASPEN Plus® were performed based on solvent-dependent experimental data (product titer, yield and productivity) to consider the physiological response of the microorganism in specific extractive ABE fermentations, and to quantify the energy requirements and the economic improvement of the overall process. Four scenarios, based on two different solvents (2-butyl-1-octanol, 2B1O, and a vegetable oil, VO) applied in batch or fed-batch operation, were compared with the batch conventional process. Total energy demand decreased in all extractive configurations and the greatest energy savings (61%) were reached with the VO-based fed-batch operation. However, the highest profit increase was achieved with 2B1O in fed-batch mode, reducing the minimum butanol selling price by 29% over the base case, along with 34% savings in raw materials and 80% wastewater reduction. The techno–economical solvent-based comparative evaluation is a useful tool to identify key challenges to be tackled when revisiting ABE extractive fermentation.
Graphical abstract
中文翻译：
丙酮-丁醇-乙醇 (ABE) 提取发酵的能源需求和经济性：基于溶剂的比较评估。
摘要
丙酮-丁醇-乙醇 (ABE) 发酵的再工业化因其巨大的生产成本而受到阻碍，这与高产物抑制和低产物收率有关。ABE 发酵可以通过整合原位液-液萃取得到显着增强。在本研究中，使用 Excel ®和 ASPEN Plus ® 的混合模拟基于溶剂相关的实验数据（产品滴度、产量和生产力）进行分析，以考虑微生物在特定提取 ABE 发酵中的生理反应，并量化整个过程的能量需求和经济改进。基于两种不同溶剂（2-丁基-1-辛醇，2B1O 和植物油，VO）在分批或补料分批操作中应用的四种方案与分批常规工艺进行了比较。所有萃取配置的总能源需求均下降，基于 VO 的分批补料操作实现了最大的节能 (61%)。然而，2B1O 在补料分批模式下实现了最高的利润增长，与基本情况相比，丁醇的最低销售价格降低了 29%，同时原材料节省了 34%，废水减少了 80%。
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