Abstract Anthropogenic activities such as oil exploration have resulted in an environmental concern as they are comprised of residual hydrocarbons and metals. Following the hypothesis that endogenous bacterial communities have enhanced tolerance to heavy metals, we isolated and characterized culturable lead-resistant bacteria from an oil wastewater sample and determined whether they could reduce lead ions from the medium. The wastewater sample containing indigenous bacteria were taken out from a traditional oil field, Bojonegoro District, East Java, Indonesia, and bacteria were cultured Halomonas complex (HMC) medium containing lead (II) chloride (PbCl2) with different concentrations. Bioaccumulation of lead by heavy-metals resistant bacteria was determined by using atomic absorption spectrophotometry (AAS). Our result found 21 bacterial strains that resist lead ions, of which one strain (RPb5-3) highly resisted to 10 mM. This bacterial strain also exhibited the highest accumulation of Pb, and it could grow at various temperatures, or more than their original environment. The bacterial strains could be used for bioremediation of lead toxicity, especially in oil pollutants.

中文翻译：

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从石油废水样品中分离出的抗铅细菌用于铅的生物修复

摘要石油勘探等人类活动含有残余碳氢化合物和金属，因此引起了环境问题。根据内源细菌群落增强对重金属耐受性的假设，我们从石油废水样品中分离和表征了可培养的抗铅细菌，并确定它们是否可以减少培养基中的铅离子。含有本土细菌的废水样品取自印度尼西亚东爪哇省Bojonegoro区的一个传统油田，在含有不同浓度氯化铅(II)(PbCl2)的盐单胞菌复合体(HMC)培养基中培养细菌。使用原子吸收分光光度法（AAS）测定重金属抗性细菌对铅的生物积累。我们的结果发现了 21 种细​​菌菌株对铅离子有抵抗力，其中一株菌株 (RPb5-3) 的抵抗力高达 10 mM。该菌株还表现出最高的铅积累量，并且可以在各种温度下生长，或者比其原始环境温度更高。该细菌菌株可用于铅毒性的生物修复，特别是石油污染物。