BACKGROUND Nyssorhynchus deaneorum is a potential malaria vector because it has been shown to be competent to transmit Plasmodium vivax and Plasmodium falciparum, and because it exhibits antropophilic and endophilic behaviors in some regions of the Amazon. This profile makes Ny. deaneorum a useful mosquito for experiments that model Plasmodium-vector interactions in the Amazon. OBJECTIVE Herein we describe how a free-mating colony of Ny. deaneorum has been established using an automated light stimulation system. METHODS Mosquitoes were captured in São Francisco do Guaporé, Rondônia. The F1 generation was reared until adult emergence at which point copulation was induced using an automatic copulation induction system (ACIS). FINDINGS After four generations, natural mating and oviposition began to occur without light stimulation. The number of pupae and adult mosquitoes increased from the F5 to F10 generations. The new Ny. deaneorum colony exhibited susceptibility to P. vivax. MAIN CONCLUSIONS Automated light stimulation is an effective method for establishing an Ny. deaneorum colony under laboratory conditions as it produces enough adults to create a stenogamic colony. The establishment of a stable, P. vivax-susceptible colony of Ny. deaneorum makes it possible to model parasite-vector interactions and to test novel drug therapies that target parasite development in mosquitoes.

中文翻译：

---

用于建立来自巴西的Nyssorhynchus deaneorum的自由交配实验室菌落的自动交配感应系统的说明。

背景技术Nyssorhynchus deaneorum是一种潜在的疟疾媒介，因为它已被证明具有传播间日疟原虫和恶性疟原虫的能力，并且在亚马逊河的某些地区表现出嗜嗜性和嗜嗜性行为。这个个人资料使Ny。deaneorum是一种有用的蚊子，可用于在亚马逊河中模拟疟原虫-媒介相互作用的实验。目的本文描述了Ny的自由交配菌落。已使用自动光刺激系统建立了脱皮。方法蚊子是在朗多尼亚州的瓜瓜雷圣弗朗西斯科州捕获的。饲养F1代直到成年出现，然后使用自动交配诱导系统（ACIS）诱导交配。发现经过四代人，自然交配和排卵开始没有光刺激。the和成年蚊子的数量从F5世代增加到F10代。新纽约州。Deaneorum菌落表现出对间日疟原虫的敏感性。主要结论自动光刺激是建立Ny的有效方法。实验室条件下的deaneorum菌落，因为它可以产生足够多的成年动物，以创建一个stenogamic菌落。建立一个稳定的，对间日疟原虫敏感的Ny菌落。deaneorum使对寄生虫-载体相互作用进行建模并测试针对蚊子中寄生虫发展的新型药物疗法成为可能。实验室条件下的deaneorum菌落，因为它可以产生足够多的成年动物，以创建一个stenogamic菌落。建立一个稳定的，对间日疟原虫敏感的Ny菌落。deaneorum使对寄生虫-载体相互作用进行建模并测试针对蚊子中寄生虫发展的新型药物疗法成为可能。实验室条件下的deaneorum菌落，因为它可以产生足够多的成年动物，以创建一个stenogamic菌落。建立一个稳定的，对间日疟原虫敏感的Ny菌落。deaneorum使对寄生虫-载体相互作用进行建模并测试针对蚊子中寄生虫发展的新型药物疗法成为可能。