Laccases have emerged as environment-friendly multifaceted biocatalysts for diverse biotechnological applications. Here, we isolated a high molecular weight (88 kDa) extremophilic laccase (LacT) from Brevibacillus agri, with the aim to exploit its extreme characters in denim bleaching. LacT has been characterized as a thermostable, acidophilic enzyme with high salt, organic solvent, and divalent metal tolerance properties. Denim bleaching efficiency of LacT was optimum at pH 4.0 and appeared to be surpassing over other reported laccases. LacT also exhibited remarkable efficacy in the decolorization of water-soluble health hazardous azo-dyes, and thus transpired to be a promising bio-bleaching and dye decolorizing agent.

漆酶已成为用于各种生物技术应用的环境友好型多方面生物催化剂。在这里，我们从农业短杆菌中分离出一种高分子量 (88 kDa) 嗜极漆酶 (LacT) ，旨在利用其在牛仔布漂白中的极端特性。LacT 已被表征为具有高盐、有机溶剂和二价金属耐受特性的热稳定、嗜酸酶。LacT 的牛仔布漂白效率在 pH 值为 4.0 时最佳，似乎超过了其他报道的漆酶。LacT 在水溶性对健康有害的偶氮染料的脱色方面也表现出显着的功效，因此被证明是一种很有前途的生物漂白和染料脱色剂。