The drug discovery rate is dramatically decreasing due to the rediscovery of known compounds. Genome mining approaches have revealed that a large portion of the actinobacterial genome that encodes bioactive metabolites is cryptic and not expressed under standard lab conditions. In the present study, we aimed to induce antibiotic encoding biosynthetic genes in a member of Micrococcales as a new species of Promicromonospora, Promicromonospora kermanensis, by chemical and biological elicitors as it was considered to produce numerous valuable bioactive metabolites based on the whole genome results. Induction has been done via chemical (antibiotics, histone deacetylase inhibitors (HDAIs), rare earth elements (REEs), fatty acid synthesis inhibitors, and extreme pH changes) and biological elicitors (live and dead Gram-positive and negative bacteria). The results showed that valproic acid (as HDAIs), DMSO, lanthanum chloride (as REE), triclosan (as fatty acid synthesis inhibitors), alkaline pH, and supernatant of Pseudomonas aeruginosa UTMC 1404 culture could act as stimuli to provoke antibacterial synthetic pathways in Promicromonospora kermanensis DSM 45485. Moreover, it was revealed that eliciting agents in cell filtrated of P. aeruginosa culture is resistant to detergent, acidic, and basic condition and have amphipathic nature. The inducing effect of alkaline pH on metabolite induction of Actinobacteria was first reported in this study. In the follow-up studies, the induced antibacterial producing clusters can be subjected to the characterization, and the implemented approach in this study can be used for metabolites induction in other selected species.

由于已知化合物的重新发现，药物发现率急剧下降。基因组挖掘方法表明，编码生物活性代谢物的大部分放线菌基因组是隐蔽的，在标准实验室条件下不表达。在本研究中，我们的目的是诱导编码抗生素生物合成基因中的一员Micrococcales作为一个新的物种原小，原小kermanensis, 被化学和生物诱导剂认为是基于全基因组结果产生许多有价值的生物活性代谢物。诱导是通过化学物质（抗生素、组蛋白去乙酰化酶抑制剂 (HDAI)、稀土元素 (REE)、脂肪酸合成抑制剂和极端 pH 变化）和生物诱导物（活的和死的革兰氏阳性菌和阴性菌）完成的。结果表明，丙戊酸（作为 HDAIs）、DMSO、氯化镧（作为 REE）、三氯生（作为脂肪酸合成抑制剂）、碱性 pH 值和铜绿假单胞菌UTMC 1404 培养物的上清液可以作为刺激物激发抗菌合成途径。Promicromonospora kermanensis DSM 45485。此外，发现细胞中的诱导剂过滤了铜绿假单胞菌培养物耐洗涤剂、酸性和碱性条件，并具有两亲性。本研究首次报道了碱性 pH 对放线菌代谢产物诱导的诱导作用。在后续研究中，可以对诱导产生的抗菌簇进行表征，本研究中实施的方法可用于其他选定物种的代谢物诱导。