Real-time fluorescence detection of nucleic acid exhibit excellent performance in analytical and diagnostic applications. However, the requirement of laboratory-based instrument and complex nucleic acid extraction greatly limits their application in point-of-care testing (POCT). Herein, a novel integrated silica membrane–based platform incorporating nucleic acid purification, amplification, and detection steps was developed. A universal and portable visualization platform was fabricated by incorporating denaturation bubble-mediated strand exchange amplification (SEA) reaction with silica membrane. The fluorescence signal of SYBR Green I with amplification products was visualized by the naked eye using a simple ultraviolet light on the silica membrane, and significant discrimination between the positive and negative samples could be easily and visually obtained. Besides, chitooligosaccharide-modified silica membrane allows the purification of nucleic acid in a totally aqueous system and enables in situ SEA. With the proposed integrated platform, 10²–10⁸ cfu/mL Vibrio parahaemolyticus could be successfully detected and excellent performance was also revealed for gram-positive pathogens. The detection limit of the method for artificially spiked oysters was 10³ cfu/g and reached 10⁰ cfu/g after 12 h enrichment. This proof-of-concept method could also be applied to a variety of nucleic acid amplification methods. We believe that the proposed silica membrane–based platform has great potential for the rapid and low-cost detection of nucleic acids especially in low-resource settings.

核酸的实时荧光检测在分析和诊断应用中表现出优异的性能。然而，基于实验室的仪器和复杂的核酸提取的要求极大地限制了它们在即时检测（POCT）中的应用。在此，开发了一种新的基于集成硅胶膜的平台，包括核酸纯化、扩增和检测步骤。通过将变性气泡介导的链交换扩增 (SEA) 反应与硅胶膜结合，制造了一个通用的便携式可视化平台。SYBR Green I 与扩增产物的荧光信号通过硅胶膜上的简单紫外光通过肉眼可视化，并且可以轻松直观地获得正样本和负样本之间的显着区分。此外，壳寡糖改性硅胶膜允许在全水系统中纯化核酸并实现原位 SEA。使用建议的集成平台，10可以成功检测到² –10 ⁸  cfu/mL副溶血性弧菌，并且对革兰氏阳性病原体也显示出优异的性能。该方法对人工添加牡蛎的检出限为10 ³  cfu/g， 富集12 h后达到10 ⁰ cfu/g。这种概念验证方法也可以应用于各种核酸扩增方法。我们相信，所提出的基于硅胶膜的平台在核酸的快速和低成本检测方面具有巨大的潜力，尤其是在资源匮乏的环境中。