Soil salinity, depending on its intensity, drives a challenged plant either to death, or survival with compromised productivity. On exposure to moderate salinity, plants can often survive by sacrificing some of their cells ‘in target’ following a route called programmed cell death (PCD). In animals, PCD has been well characterised, and involvement of mitochondria in the execution of PCD events has been unequivocally proven. In plants, mechanistic details of the process are still in grey area. Previously, we have shown that in green tissues of rice, for salt induced PCD to occur, the presence of active chloroplasts and light are equally important. In the present work, we have characterised the chloroplast proteome in rice seedlings at 12 and 24 h after salt exposure and before the time point where the signature of PCD was observed. We identified almost 100 proteins from chloroplasts, which were divided in to 11 categories based on the biological functions in which they were involved. Our results concerning the differential expression of chloroplastic proteins revealed involvement of some novel candidates. Moreover, we observed maximum phosphorylation pattern of chloroplastic proteins at an early time point (12 h) of salt exposure.

土壤盐分取决于其强度，使受挑战的植物死亡或存活而生产力受到损害。在暴露于中等盐度的情况下，植物通常可以通过遵循称为程序性细胞死亡（PCD）的途径牺牲其某些“靶”细胞来生存。在动物中，PCD具有良好的特征，线粒体参与执行PCD事件已得到明确证明。在工厂中，该过程的机械细节仍处于灰色区域。以前，我们已经证明，在水稻的绿色组织中，对于盐诱导的PCD的发生，活性叶绿体和光的存在同样重要。在目前的工作中，我们表征了水稻幼苗在盐接触后12和24 h以及观察到PCD签名的时间点之前的叶绿体蛋白质组。我们从叶绿体中鉴定了近100种蛋白质，根据它们所涉及的生物学功能将其分为11类。我们关于叶绿体蛋白差异表达的研究结果揭示了一些新型候选基因的参与。此外，我们在盐接触的早期时间点（12小时）观察到了叶绿体蛋白的最大磷酸化模式。