A high-throughput assay to quantify protein hydrolysis in aerobic and anaerobic wastewater treatment processes.,Applied Microbiology and Biotechnology

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A high-throughput assay to quantify protein hydrolysis in aerobic and anaerobic wastewater treatment processes.
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2020-07-22 , DOI: 10.1007/s00253-020-10751-4
Pieter Van Gaelen ₁ , Dirk Springael ₂ , Ilse Smets ₁

Affiliation

Abstract

Proteins, an important fraction of the organic matter in wastewater, typically enter a treatment facility as high molecular weight components. These components are degraded by extracellular protein hydrolytic enzymes, denoted as proteases. Adequate protein hydrolysis monitoring is crucial, since protein hydrolysis is often a rate-limiting step in wastewater treatment. However, current monitoring tools lack a high sample throughput and reliable quantification. Here, we present an improved assay for high-throughput protein hydrolysis rate measurements in wastewater treatment applications. A BODIPY FL casein model substrate was implemented in a microplate format for continuous fluorescent quantification. Case studies on a conventional and a high-rate aerobic municipal wastewater treatment plant and a lab-scale, two-stage, anaerobic reactor provided proof-of-concept. The assay presented in this study can help to obtain monitoring-based process insights, which will in turn allow improving biological performance of wastewater treatment installations in the future.

Key points

• Protein hydrolysis is a crucial step in biological wastewater treatment.

• Quantification of the protein hydrolysis rate enables in-depth process knowledge.

• BODIPY FL casein is a suitable model substrate for a protein hydrolysis assay.

• High sample throughput was obtained with fluorescent hydrolysis quantification.

中文翻译：

一种高通量测定法，用于量化好氧和厌氧废水处理过程中蛋白质的水解。

摘要

蛋白质是废水中有机物的重要组成部分，通常以高分子量成分的形式进入处理设备。这些成分被称为蛋白酶的细胞外蛋白水解酶降解。充分的蛋白质水解监测至关重要，因为蛋白质水解通常是废水处理中的限速步骤。然而，当前的监测工具缺乏高样品通量和可靠的定量。在这里，我们为废水处理应用中的高通量蛋白质水解速率测量提出了一种改进的测定方法。BODIPY FL酪蛋白模型底物以微孔板形式实现，用于连续荧光定量。常规和高速率好氧市政废水处理厂以及实验室规模，两阶段，厌氧反应器提供了概念验证。本研究中介绍的测定法可以帮助获得基于监控的过程见解，从而可以在将来改善废水处理装置的生物学性能。