An effective strategy of hyperthermia-chemotherapy-regeneration for bone-related cancer treatment is presented. For this purpose, a new approach of magnetic particles (MPs) encapsulated in bioactive glass (BG) structure, with anti-cancer activity, is evaluated. MPs are initially synthesized using a co-precipitation method and then embedded into BG structure through a sol–gel synthesis process. Results confirmed the formation of a crystalline and pure MP structure. MP-BG particles were found to be bioactive by forming a hydroxyapatite layer on their surface. The hyperthermia application of a MP-BG system was also studied. It was found that the particles reach a temperature of 42 C in an alternating magnetic field. Doxorubicin (DOX), a widely used anticancer drug, was loaded in MP-BG. To enhance the loading efficiency, the BG was surface modified to create NH₂ groups on the surface. The encapsulation and release of DOX was studied over 48 h. In vitro tests were performed using human osteosarcoma cell line (MG63). The results demonstrated the non-cytotoxic nature of MP and MP-BG tested at various concentrations. DOX release from MP-BG resulted in decreased MG63 viability. Also, fluorescence microscopy visualization confirmed the intracellular uptake of MP-BG particles and the release of DOX. These results indicate that our suggested strategy of combined hyperthermia-chemotherapy-regeneration using MP-BG structure represents a powerful approach in cancer treatment and tissue regeneration.

提出了一种有效的热疗-化疗-再生治疗骨相关癌症的策略。为此，评估了一种封装在生物活性玻璃 (BG) 结构中的磁性颗粒 (MPs) 具有抗癌活性的新方法。MPs 最初使用共沉淀法合成，然后通过溶胶-凝胶合成工艺嵌入 BG 结构中。结果证实了结晶和纯 MP 结构的形成。通过在其表面形成羟基磷灰石层，发现 MP-BG 颗粒具有生物活性。还研究了 MP-BG 系统的热疗应用。发现颗粒在交变磁场中达到 42 摄氏度的温度。多柔比星 (DOX) 是一种广泛使用的抗癌药物，被加载到 MP-BG 中。为了提高装载效率，₂组表面。在 48 小时内研究了 DOX 的封装和释放。使用人骨肉瘤细胞系（MG63）进行体外试验。结果证明了在不同浓度下测试的 MP 和 MP-BG 的非细胞毒性性质。MP-BG 释放 DOX 导致 MG63 活力降低。此外，荧光显微镜观察证实了 MP-BG 颗粒的细胞内摄取和 DOX 的释放。这些结果表明，我们建议的使用 MP-BG 结构的热疗-化疗-再生联合策略代表了癌症治疗和组织再生的有力方法。