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Structural Basis of Hydrogenotrophic Methanogenesis.
Annual Review of Microbiology ( IF 8.5 ) Pub Date : 2020-09-09 , DOI: 10.1146/annurev-micro-011720-122807
Seigo Shima 1 , Gangfeng Huang 1 , Tristan Wagner 2 , Ulrich Ermler 3
Affiliation  

Most methanogenic archaea use the rudimentary hydrogenotrophic pathway—from CO2 and H2 to methane—as the terminal step of microbial biomass degradation in anoxic habitats. The barely exergonic process that just conserves sufficient energy for a modest lifestyle involves chemically challenging reactions catalyzed by complex enzyme machineries with unique metal-containing cofactors. The basic strategy of the methanogenic energy metabolism is to covalently bind C1 species to the C1 carriers methanofuran, tetrahydromethanopterin, and coenzyme M at different oxidation states. The four reduction reactions from CO2 to methane involve one molybdopterin-based two-electron reduction, two coenzyme F420–based hydride transfers, and one coenzyme F430–based radical process. For energy conservation, one ion-gradient-forming methyl transfer reaction is sufficient, albeit supported by a sophisticated energy-coupling process termed flavin-based electron bifurcation for driving the endergonic CO2 reduction and fixation. Here, we review the knowledge about the structure-based catalytic mechanism of each enzyme of hydrogenotrophic methanogenesis.

中文翻译:


氢营养型甲烷生成的结构基础。

多数产甲烷古细菌使用基本的氢营养途径(从CO 2和H 2到甲烷)作为缺氧生境中微生物生物量降解的最终步骤。仅能节省适度生活方式所需的足够能量的过程,涉及复杂的酶机制与独特的含金属辅因子催化的化学挑战性反应。产甲烷能量代谢的基本策略是将C 1物种与C 1载体在不同氧化态下与甲氧呋喃,四氢甲蝶呤和辅酶M共价结合。从CO 2到甲烷的四个还原反应涉及一个基于钼钼素的双电子还原,两个辅酶F 420氢化物转移和一种基于辅酶F 430的自由基过程。为了节约能源,一个离子梯度形成的甲基转移反应就足够了,尽管有一种复杂的能量耦合方法(称为黄素类电子分叉法)来支持驱使负电子CO 2还原和固定。在这里,我们审查有关氢营养型甲烷生成的每种酶的基于结构的催化机理的知识。

更新日期:2020-09-10
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