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SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro.
Memórias do Instituto Oswaldo Cruz ( IF 2.5 ) Pub Date : 2020-07-13 , DOI: 10.1590/0074-02760200200
Marcio da Costa Cipitelli 1 , Elizabeth Valentin 2 , Nadia Vaez Gonçalves da Cruz 2 , Tatiana Ls Nogueira 3 , Elaine Cristina Amaro de Melo 1 , Rebeca Araujo da Silva 1 , Marcelo M Serra 2 , André L Meriano 1 , Alberto Ml Colares 2 , Marcos Dornelas-Ribeiro 1 , Caleb Gm Santos 1
Affiliation  

Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.

中文翻译:


SARS-CoV-2 诊断日记:从谣言到首例病例。里约热内卢军事研究和诊断研究所的分子测试早期报告。



冠状病毒病(COVID-19)对全球健康构成严重威胁。提出了政府警报(1 月 22 日)(D)中早期分子诊断的历史时间表。经过计算机分析后,巴西陆军生物研究所 (IBEx-RJ) 根据疾病控制和预防中心 (CDC) 的建议,使用实时逆转录酶聚合酶链反应 (RT-PCR)(快速模式)在内部测试了样本。巴西、里约热内卢、IBEx 和诊断团队分别于第 36 日、第 44 日、第 66 日和第 74 日报告了首例病例。因此,经过 1300 次测试后,如果出现阳性结果,我们建议使用 N1/N2 引物组 (CDC) 进行初步和 Charité 方案确认。此外,除了每周对所有相关人员进行测试外,每个专业人员在开始工作前都应该接受测试。
更新日期:2020-08-20
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