Abstract Transcription factors play an important role in fungal environmental adaptive process by promoting adjustment to challenging stimuli via gene modulation and activation of signaling networks. The transcription factor encoded by the pac-3/rim101/pacC gene is involved in pH regulation and is associated with a wide variety of cellular functions. The deletion of pac-3 affects fungal development. In Neurospora crassa, the Δpac-3 strain presents diminished aerial growth and reduced conidiation. However, the PAC-3-regulated genes associated with this altered phenotype have not been elucidated. In this study, we used RNA-seq to analyze the phenotypic plasticity induced after pac-3 deletion in the filamentous fungus N. crassa cultivated in media supplemented with sufficient or limited inorganic phosphate. Genes related to morphology, hyphal development, and conidiation were of particular interest in this study. Our results suggest a pac-3 dependency in gene regulation in a Pi-dependent manner. Furthermore, our analysis suggested that the fungus attempts to overcome the deletion effects in a Δpac-3 mutant through a complex combined regulatory mechanism. Finally, the modulatory responses observed in the Δpac-3 strain, a double mutant generated based on the Δmus-52 mutant strain, is strain-specific, highlighting that the phenotypic impact may be attributed to pac-3 absence despite the combined mus-52 deletion.

中文翻译：

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PAC-3 转录因子严重调控粗糙脉孢菌中的表型相关基因

摘要 转录因子通过基因调节和信号网络激活促进对挑战性刺激的适应，在真菌环境适应过程中发挥重要作用。由 pac-3/rim101/pacC 基因编码的转录因子参与 pH 调节并与多种细胞功能相关。pac-3 的缺失会影响真菌的发育。在粗糙脉孢菌中，Δpac-3 菌株表现出减少的空中生长和减少的分生孢子。然而，与这种改变的表型相关的 PAC-3 调节基因尚未阐明。在本研究中，我们使用 RNA-seq 分析了在补充有足够或有限无机磷酸盐的培养基中培养的丝状真菌 N. crassa 中 pac-3 缺失后诱导的表型可塑性。与形态相关的基因，本研究对菌丝发育和分生孢子形成特别感兴趣。我们的结果表明 pac-3 依赖于 Pi 依赖性方式的基因调控。此外，我们的分析表明，真菌试图通过复杂的组合调节机制克服 Δpac-3 突变体中的缺失效应。最后，在 Δpac-3 菌株（基于 Δmus-52 突变菌株产生的双突变体）中观察到的调节反应是菌株特异性的，突出表明尽管结合了 mus-52，表型影响可能归因于 pac-3 的缺失删除。我们的分析表明，真菌试图通过复杂的组合调节机制克服 Δpac-3 突变体中的缺失效应。最后，在 Δpac-3 菌株（基于 Δmus-52 突变菌株产生的双突变体）中观察到的调节反应是菌株特异性的，突出表明尽管结合了 mus-52，表型影响可能归因于 pac-3 的缺失删除。我们的分析表明，真菌试图通过复杂的组合调节机制克服 Δpac-3 突变体中的缺失效应。最后，在 Δpac-3 菌株（基于 Δmus-52 突变菌株产生的双突变体）中观察到的调节反应是菌株特异性的，突出表明尽管结合了 mus-52，表型影响可能归因于 pac-3 的缺失删除。